Recombinant Proteins
Product List
Der F1
Der F1 Mosaic Protein Recombinant
Der P1
Der P1 Protein Recombinant
The E.Coli derived recombinant protein contains the Dermatophagoides pteronyssinus Dust Mite Der P1 protein (a.a. 20-320) and fused to a 6 His Tag at C-terminus, having a total Mw of 34.5kDa, pI 5.6.
Fra e 1.0101
Allergen Fra e 1.0101 Recombinant
Fra e 1.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Gly m 4.0101
Stress-Induced Protein SAM22 Recombinant
Gly m 4.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Gly m 5.0101
Alpha Subunit of Beta Conglycinin Recombinant
Gly m 5.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Glycinin
Allergen Ara h 3.0101 Recombinant
Recombinant Glycinin produced in E. coli is a non- glycosylated, polypeptide chain having a calculated molecular mass of 63 kDa.
Glycinin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
MALD1
Major Allergen Mal d 1 Recombinant (Mal d 1.0108)
Recombinant MALD1 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 17,492 Dalton.
MALD1 purified by proprietary chromatographic techniques.
MALD3
Non-Specific Lipid-Transfer Protein Mal d 3 Recombinant
Recombinant Non-Specific Lipid-Transfer Protein Mal d 3 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 12kDa.
MALD3 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Pen a 1.0101
Tropomyosin Pen a 1.0101 Recombinant
Recombinant Tropomyosin Pen a 1.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 38kDa.
Pen a 1.0101 is expressed with a 10xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
PhI p 1
Pollen allergen Phl p 1 Recombinant
PhI p 1 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction
Allergy is an immune system response to a foreign substance (allergen) that is not typically harmful to the body. These foreign substances can include pollen, pet dander, certain foods, and insect stings. Allergies are classified into several types based on the mechanism of the immune response:
- Type I (Immediate Hypersensitivity): Mediated by IgE antibodies, leading to reactions such as hay fever, asthma, and anaphylaxis.
- Type II (Cytotoxic Hypersensitivity): Involves IgG or IgM antibodies targeting cells, leading to conditions like hemolytic anemia.
- Type III (Immune Complex Hypersensitivity): Involves immune complexes that deposit in tissues, causing conditions like systemic lupus erythematosus.
- Type IV (Delayed-Type Hypersensitivity): Mediated by T cells, leading to reactions such as contact dermatitis and tuberculin reactions.
- Key Biological Properties: Allergies involve the immune system’s overreaction to harmless substances. Key players include IgE antibodies, mast cells, basophils, and various cytokines.
- Expression Patterns: IgE antibodies are produced by B cells and are specific to particular allergens. Mast cells and basophils express high-affinity IgE receptors (FcεRI).
- Tissue Distribution: Mast cells are found in connective tissues, particularly near blood vessels and nerves, while basophils circulate in the blood.
- Primary Biological Functions: The primary function of the allergic response is to protect the body from perceived threats. This involves the release of histamines and other mediators that cause inflammation and other symptoms.
- Role in Immune Responses: Allergies play a role in immune responses by activating various immune cells and mediators.
- Pathogen Recognition: Although allergies are typically responses to non-pathogenic substances, the mechanisms involved are similar to those used in pathogen recognition.
- Mechanisms with Other Molecules and Cells: Allergens bind to IgE antibodies on the surface of mast cells and basophils, leading to cell activation and degranulation.
- Binding Partners: Key binding partners include IgE antibodies, FcεRI receptors, and allergens.
- Downstream Signaling Cascades: Activation of mast cells and basophils leads to the release of histamines, leukotrienes, and other inflammatory mediators, triggering symptoms like itching, swelling, and bronchoconstriction.
- Regulatory Mechanisms: The expression and activity of IgE and other molecules involved in allergic responses are tightly regulated by various factors.
- Transcriptional Regulation: Genes encoding IgE and FcεRI are regulated by transcription factors such as NF-κB.
- Post-Translational Modifications: Post-translational modifications, such as phosphorylation, play a role in the activation and function of immune cells involved in allergies.
- Biomedical Research: Understanding allergies helps in developing treatments and preventive measures.
- Diagnostic Tools: Skin prick tests, blood tests for specific IgE, and challenge tests are used to diagnose allergies.
- Therapeutic Strategies: Treatments include antihistamines, corticosteroids, and immunotherapy (allergy shots).
- Throughout the Life Cycle: Allergies can develop at any age and may change over time. They can affect quality of life and may be associated with other conditions such as asthma and eczema.
- From Development to Aging and Disease: Allergies can impact various stages of life, from childhood to old age, and may influence the development of other immune-related diseases.
