Recombinant Proteins
Product List
POU2AF1 Human
POU class 2 associating factor 1 Human Recombinant
The POU2AF1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
POU5F1 Human
POU Class 5 Homeobox 1 Human Recombinant
POU5F1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
POU5F1 Human, PolyR
POU Class 5 Homeobox 1 Human Recombinant, Polyarginine-Tag
The POU5F1 is fused to a C-terminal poly-arginine tag and purified by proprietary chromatographic techniques.
POU6F1 Human
POU Class 6 Homeobox 1 Human Recombinant
POU6F1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
The POU class represents a group of transcription factors characterized by the presence of a bipartite DNA-binding domain known as the POU domain. This domain is composed of a POU-specific domain (POU S) and a homeodomain (POU HD), connected by a flexible linker. The name “POU” is derived from the names of three transcription factors: Pit-1, Oct-1, and Oct-2, as well as the Caenorhabditis elegans gene unc-86 . POU transcription factors are classified into six major classes (POU1-POU6), each with distinct roles in various biological processes .
POU transcription factors are known for their roles in neurogenesis, pluripotency, and cell-type specification . They are expressed in a variety of tissues, including the nervous system, immune system, and epithelial tissues . For example, POU2F3 is a master regulator of tuft cell fate in the gastrointestinal and respiratory tracts . The expression patterns of POU genes are tightly regulated and often tissue-specific, reflecting their diverse biological functions .
POU transcription factors play crucial roles in various biological functions. They are involved in the regulation of gene expression during development, maintenance of pluripotency in stem cells, and differentiation of specific cell types . For instance, Oct4 (Pou5f1) is essential for maintaining the pluripotency of embryonic stem cells and is a key factor in somatic cell reprogramming . Additionally, POU factors are involved in immune responses and pathogen recognition, as they regulate the expression of genes critical for these processes .
POU transcription factors exert their effects by binding to specific DNA sequences and interacting with other proteins to regulate gene expression . The POU domain allows these factors to bind to DNA with high specificity and affinity. They can form homodimers or heterodimers, which enhances their ability to regulate target genes . POU factors also interact with other transcription factors and co-regulators to modulate downstream signaling cascades, influencing various cellular processes .
The expression and activity of POU transcription factors are tightly regulated at multiple levels. Transcriptional regulation involves the binding of other transcription factors to the promoter regions of POU genes . Post-translational modifications, such as phosphorylation and ubiquitination, also play a crucial role in modulating the stability, localization, and activity of POU proteins . These regulatory mechanisms ensure that POU factors are expressed at the right time and place, allowing them to perform their functions effectively .
POU transcription factors have significant applications in biomedical research, diagnostics, and therapeutics. In research, they are used to study gene regulation, development, and cell differentiation . Oct4, for example, is a marker for pluripotent stem cells and is used in stem cell research and regenerative medicine . In diagnostics, POU factors can serve as biomarkers for certain diseases, such as cancer . Therapeutically, targeting POU factors or their regulatory pathways holds potential for treating various conditions, including cancer and neurodegenerative diseases .
POU transcription factors play vital roles throughout the life cycle, from development to aging and disease . During early development, they are essential for the activation of the zygotic genome and the maintenance of pluripotency . In adulthood, POU factors continue to regulate the differentiation and function of various cell types . Dysregulation of POU factors has been linked to aging and the development of diseases, highlighting their importance in maintaining cellular homeostasis .
