Recombinant Proteins
Product List
Cadherin-E Human
Cadherin-E Human Recombinant
The Cadherin-E is purified by proprietary chromatographic techniques.
CDH1 Human, HEK
E-Cadherin Human Recombinant, HEK
CDH1 Human, Sf9
E-Cadherin Human Recombinant, Sf9
CDH11 Human
Cadherin 11 Human Recombinant
CDH2 Human
Cadherin 2 Human Recombinant
CDH2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 574 amino acids (160-724a.a.) and having a molecular mass of 62.9kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CDH2 is expressed with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
CDH5 Human
Cadherin 5 Human Recombinant
CDH5 Mouse
Cadherin 5 Mouse Recombinant
CDH5 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 583 amino acids (25-599a.a.) and having a molecular mass of 66.2kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
CDH5 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
CDHR5 Mouse
Cadherin Related Family Member 5 Mouse Recombinant
CDHR5 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 621 amino acids (29-641 a.a) and having a molecular mass of 66.3kDa.
CDHR5 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Cadherins are a class of type-1 transmembrane proteins that play a crucial role in cell-cell adhesion. They are calcium-dependent adhesion molecules, meaning their adhesive function is regulated by the presence of calcium ions . Cadherins are classified into several types based on their structure and function:
- Classical Cadherins: These include E-cadherin, N-cadherin, and P-cadherin, which are primarily involved in maintaining cell-cell adhesion in epithelial, neural, and placental tissues, respectively .
- Desmosomal Cadherins: These include desmogleins and desmocollins, which are involved in forming desmosomes that provide mechanical strength to tissues .
- Protocadherins: These are primarily involved in neural development and function .
- Atypical Cadherins: These include molecules like CELSR1, which are involved in transmitting developmental signals rather than adhesion .
Cadherins exhibit several key biological properties:
- Expression Patterns: Cadherins are expressed in a tissue-specific manner. For example, E-cadherin is predominantly found in epithelial tissues, while N-cadherin is expressed in neural and mesenchymal tissues .
- Tissue Distribution: Cadherins are widely distributed across various tissues, including epithelial, neural, and endothelial tissues .
- Key Biological Properties: Cadherins mediate cell-cell adhesion, maintain tissue architecture, and regulate cell polarity and migration .
Cadherins serve several primary biological functions:
- Cell-Cell Adhesion: Cadherins mediate homophilic cell-cell adhesion, meaning they bind to the same type of cadherin on adjacent cells .
- Tissue Morphogenesis: During development, cadherins play a critical role in tissue morphogenesis by regulating cell sorting and tissue organization .
- Immune Responses and Pathogen Recognition: Cadherins are involved in immune responses by mediating interactions between immune cells and other cell types .
Cadherins interact with other molecules and cells through several mechanisms:
- Binding Partners: Cadherins bind to catenins (α-, β-, and γ-catenins) on the intracellular side, linking them to the actin cytoskeleton .
- Downstream Signaling Cascades: Cadherin-catenin complexes can activate various signaling pathways, including the Wnt/β-catenin pathway, which regulates gene expression and cell behavior .
The expression and activity of cadherins are regulated through multiple mechanisms:
- Transcriptional Regulation: Cadherin gene expression is controlled by transcription factors and signaling pathways that respond to developmental and environmental cues .
- Post-Translational Modifications: Cadherins undergo post-translational modifications such as phosphorylation, which can modulate their adhesive activity and interactions with other proteins .
Cadherins have several applications in biomedical research and clinical practice:
- Biomedical Research: Cadherins are used as markers to study cell adhesion, tissue development, and cancer progression .
- Diagnostic Tools: Altered cadherin expression is used as a diagnostic marker for various cancers and other diseases .
- Therapeutic Strategies: Targeting cadherin interactions and signaling pathways is being explored as a therapeutic strategy for cancer and other diseases .
Cadherins play a vital role throughout the life cycle:
- Development: During embryonic development, cadherins are essential for tissue formation and organogenesis .
- Aging: Cadherin function can be altered with aging, affecting tissue integrity and function .
- Disease: Dysregulation of cadherin expression and function is associated with various diseases, including cancer and neurodegenerative disorders .
