Recombinant Proteins
Product List
NECTIN2 Human
Nectin Cell Adhesion Molecule 2 Human Recombinant
NECTIN2 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 337 amino acids (32-360a.a.) and having a molecular mass of 36.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
NECTIN2 is expressed with an 8 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
NECTIN1 Human
Nectin Cell Adhesion Molecule 1 Human Recombinant
NECTIN1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 334amino acids (31-355a.a) and having a molecular mass of 37.3kDa.
NECTIN1 is fused to an 9 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
NECTIN3 Human
Nectin Cell Adhesion Molecule 3 Human Recombinant
NECTIN3 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 355 amino acids (58-404 a.a.) and having a molecular mass of 39.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
NECTIN3 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
Sterile filtered colorless solution.
Introduction
Nectins are a family of cellular adhesion molecules that belong to the immunoglobulin superfamily. They are involved in calcium-independent cellular adhesion and play crucial roles in the formation of various cell-cell junctions . The nectin family consists of four members: nectin-1, nectin-2, nectin-3, and nectin-4 . These molecules are ubiquitously expressed and have adhesive roles in a wide range of tissues, including epithelial and neuronal tissues .
Key Biological Properties: Nectins are characterized by their ability to form both homophilic (same type) and heterophilic (different types) interactions . They have three extracellular immunoglobulin domains, a single transmembrane helix, and an intracellular domain that can bind to the scaffold protein afadin .
Expression Patterns and Tissue Distribution: Nectins are ubiquitously expressed in various tissues. For example, nectin-1 is found in the brain, skin, and eyes, while nectin-2 is expressed in the spleen, liver, and lungs . Nectin-3 is primarily found in the testis and placenta, and nectin-4 is expressed in the placenta and embryonic tissues .
Primary Biological Functions: Nectins play essential roles in cell-cell adhesion, which is critical for the formation and maintenance of tissue architecture . They are involved in the formation of adherens junctions in epithelial cells and synaptic junctions in neurons .
Role in Immune Responses and Pathogen Recognition: Nectins are involved in immune responses by mediating the adhesion of immune cells to target cells . They also play a role in pathogen recognition, as certain viruses, such as herpes simplex virus, use nectins as entry receptors .
Mechanisms with Other Molecules and Cells: Nectins interact with other cell adhesion molecules, such as cadherins, to enhance cell-cell adhesion . They can form homo-cis dimers on the same cell membrane and trans-interact with nectins on adjacent cells .
Binding Partners and Downstream Signaling Cascades: Nectins bind to afadin, which links them to the actin cytoskeleton . This interaction activates small G proteins, such as Cdc42 and Rac, which are involved in various signaling pathways that regulate cell movement, proliferation, and survival .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of nectins are regulated at multiple levels, including transcriptional regulation and post-translational modifications . Transcription factors and signaling molecules can modulate the expression of nectin genes, while post-translational modifications, such as phosphorylation, can affect their activity and interactions .
Biomedical Research: Nectins are used as markers in various biomedical research studies to understand cell adhesion and signaling mechanisms .
Diagnostic Tools: Nectin-4, in particular, has been identified as a biomarker for certain cancers, such as breast and urothelial cancers . Diagnostic tools targeting nectin-4 are being developed to detect and monitor these cancers .
Therapeutic Strategies: Nectins are being explored as therapeutic targets. For example, enfortumab vedotin, an antibody-drug conjugate targeting nectin-4, has been approved for the treatment of urothelial carcinoma .
Role Throughout the Life Cycle: Nectins play vital roles throughout the life cycle, from development to aging and disease . During development, they are involved in the formation of tissues and organs by mediating cell-cell adhesion . In adulthood, they maintain tissue integrity and function . Dysregulation of nectin expression or function can lead to various diseases, including cancer and neurological disorders .
