NECTIN1 Human
Sf9, Baculovirus cells.
PVRL1, CD111, CLPED1, ED4, HIgR, HVIS, HVEC, Nectin-1, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12.
Greater than 95.0% as determined by SDS-PAGE.
Description
NECTIN1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 334amino acids (31-355a.a) and having a molecular mass of 37.3kDa.
NECTIN1 is fused to an 9 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Nectin-1, also known as ED4 or poliovirus receptor-related 1 protein, belongs to the Nectin family. This protein facilitates cell-to-cell connections by creating symmetrical (homophilic) or asymmetrical (heterophilic) pairings across cells. Specifically, Nectin-1 can form heterophilic interactions with PVRL4 (Nectin-4) and PVRL3 (Nectin-3). Beyond its role in cell adhesion, Nectin-1 acts as a receptor for viruses like herpes simplex virus and pseudorabies virus, allowing them to enter cells. Additionally, Nectin-1 has been shown to promote the growth of neurites, which are projections from neurons.
This product consists of the human NECTIN1 protein, recombinantly produced in Sf9 insect cells (using a baculovirus expression system). It is a single polypeptide chain, containing 334 amino acids (specifically, amino acids 31-355 of the full protein sequence), and has a molecular weight of 37.3 kDa. This NECTIN1 protein is glycosylated, meaning it has sugar molecules attached to it. A 9 amino acid Histidine tag (His-tag) has been added to the C-terminus of the protein to facilitate purification, which was carried out using specialized chromatographic techniques.
This NECTIN1 solution is provided at a concentration of 1mg/ml and is prepared in a buffer consisting of Phosphate-Buffered Saline (pH 7.4) with 10% glycerol.
For short-term storage (up to 2-4 weeks), keep the product refrigerated at 4°C. For extended storage, freeze the solution at -20°C. It is highly recommended to supplement the solution with a carrier protein (like 0.1% HSA or BSA) before freezing for long-term stability. Repeated freezing and thawing of the product should be avoided.
This product exhibits a purity greater than 95%, as determined by SDS-PAGE analysis.
PVRL1, CD111, CLPED1, ED4, HIgR, HVIS, HVEC, Nectin-1, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12.
Sf9, Baculovirus cells.
ADPQVVQVND SMYGFIGTDV VLHCSFANPL PSVKITQVTW QKSTNGSKQN VAIYNPSMGV SVLAPYRERV EFLRPSFTDG TIRLSRLELE DEGVYICEFA TFPTGNRESQ LNLTVMAKPT NWIEGTQAVL RAKKGQDDKV LVATCTSANG KPPSVVSWET RLKGEAEYQE IRNPNGTVTV ISRYRLVPSR EAHQQSLACI VNYHMDRFKE SLTLNVQYEP EVTIEGFDGN WYLQRMDVKL
TCKADANPPA TEYHWTTLNG SLPKGVEAQN RTLFFKGPIN YSLAGTYICE ATNPIGTRSG QVEVNITEFP YTPSPPEHGR RAGPVPTAHH HHHH
Product Science Overview
Nectin Cell Adhesion Molecule 1, also known as Nectin-1, is a member of the nectin subfamily of immunoglobulin-like adhesion molecules. These molecules play a crucial role in calcium-independent cell-cell adhesion. Nectin-1 is encoded by the NECTIN1 gene and is involved in the formation and maintenance of adherens junctions and tight junctions in epithelial and endothelial cells .
Nectin-1 is characterized by its structure, which includes three extracellular immunoglobulin-like loops, a single transmembrane domain, and a cytoplasmic region. This structure allows Nectin-1 to form homophilic (same molecule) or heterophilic (different molecules) trans-dimers, facilitating cell-cell adhesion . Nectin-1 interacts with the actin cytoskeleton through the adaptor protein afadin, which is essential for the formation of adherens junctions .
Mutations in the NECTIN1 gene are associated with several genetic disorders, including cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) and non-syndromic cleft lip with or without cleft palate (CL/P). These mutations can disrupt the normal function of Nectin-1, leading to developmental abnormalities .
Research on Nectin-1 has revealed its importance in various biological processes. For instance, studies have shown that mice lacking Nectin-1 exhibit defective enamel formation in their incisor teeth, highlighting its role in dental development . Additionally, Nectin-1 is involved in the regulation of desmosome assembly, which is crucial for maintaining the integrity of epithelial tissues .
