Protein Expression

Introduction

Over time, THE BioTek has amassed extensive expertise in developing bacterial-derived recombinant proteins. THE BioTek's Protein services are priced based on the amount of strain comparison and optimization work required. Our pricing structure includes a setup fee for expression analysis, the number of different strains (possibly with various helper plasmid systems), and the number of different culture conditions tested. THE BioTek's purified proteins achieve over 90% purity, as determined by SDS-PAGE analysis.For detailed pricing information, please contact sales@thebiotek.com.

Services	Deliverables	Description	Timeline
Non-reimbursable setup charge to determine solubility and level of difficulty.	SDS-PAGE image, expression levels according to strains, etc….	Non-reimbursable setup charge to determine solubility and level of difficulty.	2weeks
Purified Protein	One liter expression & one purification step	Purified soluble tagged protein and prices might change according to the expression pattern	4 weeks
Non purified/Denatured	Protein is purified from IBs, supplied in buffer	Denatured protein, no chromatography steps	4 weeks
Q.C. Western Blot	Western Blot assay Image	Antibody is provided by the customer	4 weeks
Endotoxin Assay	validation of > or < 1EU/ug protein	determined by LAL method	4 weeks