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SSBP1
Single-Stranded DNA Binding Protein 1 Sulfolobus solfataricus Recombinant
Recombinant Sulfolobus solfataricus Single-Stranded DNA Binding Protein 1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 148 amino acids and having a molecular mass of 16.1kDa. The SSBP1 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs 
SSBP1 Human, His
Single-Stranded DNA Binding Protein 1 Human Recombinant, His Tag
SSBP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (17-148 a.a.) and having a molecular mass of 17kDa.
SSBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SSBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Introduction
Definition and Classification
Single-Stranded DNA Binding Proteins (SSBs) are essential proteins that bind to single-stranded DNA (ssDNA) during various cellular processes such as DNA replication, repair, and recombination. They prevent the ssDNA from forming secondary structures and protect it from nucleases. SSBs are classified based on their origin and structure:
- Prokaryotic SSBs: Found in bacteria, such as the E. coli SSB.
- Eukaryotic SSBs: Found in eukaryotic organisms, such as Replication Protein A (RPA) in humans.
- Viral SSBs: Found in viruses, such as the T4 phage gene 32 protein.
Biological Properties
Key Biological Properties:
- High Affinity for ssDNA: SSBs bind ssDNA with high affinity and specificity.
- Cooperative Binding: SSBs often bind ssDNA cooperatively, meaning the binding of one SSB facilitates the binding of additional SSBs.
Expression Patterns:
- SSBs are ubiquitously expressed in all cells due to their essential role in DNA metabolism.
Tissue Distribution:
- SSBs are found in all tissues, with higher expression in rapidly dividing cells such as those in the bone marrow and gastrointestinal tract.
Biological Functions
Primary Biological Functions:
- DNA Replication: SSBs stabilize ssDNA during replication, allowing DNA polymerases to synthesize the new strand.
- DNA Repair: SSBs protect ssDNA intermediates during repair processes.
- Recombination: SSBs facilitate the formation of the recombination complex by stabilizing ssDNA.
Role in Immune Responses and Pathogen Recognition:
- SSBs are indirectly involved in immune responses by maintaining genome stability, which is crucial for the proper functioning of immune cells.
Modes of Action
Mechanisms with Other Molecules and Cells:
- SSBs interact with various proteins involved in DNA metabolism, such as DNA polymerases, helicases, and nucleases.
Binding Partners:
- SSBs bind to ssDNA and other proteins, forming complexes that are essential for DNA replication, repair, and recombination.
Downstream Signaling Cascades:
- SSBs do not directly participate in signaling cascades but are crucial for the processes that maintain genomic integrity, which indirectly affects cellular signaling.
Regulatory Mechanisms
Regulatory Mechanisms Controlling Expression and Activity:
- Transcriptional Regulation: SSB gene expression is regulated by transcription factors that respond to cellular stress and DNA damage.
- Post-Translational Modifications: SSBs can be modified by phosphorylation, acetylation, and ubiquitination, which can affect their activity and interactions.
Applications
Biomedical Research:
- SSBs are used as tools to study DNA replication, repair, and recombination mechanisms.
Diagnostic Tools:
- SSBs can be used in assays to detect ssDNA, which is indicative of DNA damage or viral infection.
Therapeutic Strategies:
- Targeting SSBs or their interactions with other proteins can be a potential strategy for cancer therapy, as rapidly dividing cancer cells rely heavily on SSBs for DNA replication.
Role in the Life Cycle
Role Throughout the Life Cycle:
- Development: SSBs are essential for DNA replication and repair during cell division, which is crucial for development.
- Aging: The efficiency of DNA repair mechanisms, including those involving SSBs, declines with age, leading to genomic instability.
- Disease: Mutations or dysregulation of SSBs can lead to diseases such as cancer, where genomic instability is a hallmark.
