Recombinant Proteins
Product List
EDA2R Human
Ectodysplasin A2 Receptor Human Recombinant
EDA2R Human, Sf9
Ectodysplasin A2 Receptor Human Recombinant, Sf9
EDA2R Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 380 amino acids (1-138a.a.) and having a molecular mass of 42.5kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). EDA2R is expressed with a 242 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
EDAR Human
Ectodysplasin A Receptor Human Recombinant
EDAR Human, Sf9
Ectodysplasin A Receptor Human Recombinant, Sf9
EDAR produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413 amino acids (27-187a.a.) and having a molecular mass of 45.6kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
EDAR is expressed with a 249 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Sterile filtered colorless solution.
Introduction
Ectodysplasin A (EDA) is a protein encoded by the EDA gene, belonging to the tumor necrosis factor (TNF) family. It plays a crucial role in the development of ectodermal tissues such as skin, hair, teeth, and certain glands . EDA exists in two main splice variants: EDA-A1 and EDA-A2, which bind to different receptors .
Key Biological Properties: EDA is a transmembrane protein that functions as a homotrimer. It is involved in the formation of various ectodermal derivatives during prenatal development .
Expression Patterns: EDA is primarily expressed in ectodermal tissues, including skin, hair follicles, and glands .
Tissue Distribution: EDA is distributed in tissues derived from the ectoderm, such as the epidermis, hair follicles, teeth, and sweat glands .
Primary Biological Functions: EDA is essential for the morphogenesis of skin appendages, including hair, teeth, and glands . It also plays a role in the development of feathers in birds and scales in fish .
Role in Immune Responses and Pathogen Recognition: EDA signaling has been implicated in the regulation of immune responses, particularly in the context of inflammation and pathogen recognition .
Mechanisms with Other Molecules and Cells: EDA exerts its effects by binding to its receptors, EDAR and EDA2R. EDA-A1 binds to EDAR, while EDA-A2 binds to EDA2R .
Binding Partners and Downstream Signaling Cascades: Upon binding to its receptors, EDA activates the NF-κB signaling pathway through the recruitment of the EDAR-associated death domain (EDARADD) adapter protein . This signaling cascade leads to the transcription of target genes involved in the development of ectodermal tissues .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of EDA are regulated at multiple levels, including transcriptional regulation and post-translational modifications .
Transcriptional Regulation: The EDA gene is regulated by various transcription factors that control its expression during development .
Post-Translational Modifications: EDA undergoes proteolytic processing to generate its active form, which can then bind to its receptors and initiate signaling .
Biomedical Research: EDA and its signaling pathway are studied extensively in the context of developmental biology and genetic disorders .
Diagnostic Tools: Mutations in the EDA gene are associated with ectodermal dysplasias, which can be diagnosed through genetic testing .
Therapeutic Strategies: Recombinant EDA protein has been explored as a potential therapeutic strategy for treating ectodermal dysplasias .
