Recombinant Proteins
Product List
FZD10 Human
Frizzled Class Receptor 10 Human Recombinant
FZD10 Human Recombinant is a single, glycosylated, polypeptide chain (21-225 a.a) containing a total of 444 amino acids and having a molecular mass of 50.0 kDa.
FZD10is fused to a 239 a.a hIgG-His-Tag at C-terminus and is purified by proprietary chromatographic techniques.
FZD4 Human
Frizzled Class Receptor 4 Human Recombinant
FZD4 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 428 amino acids (37-222 a.a.) and having a molecular mass of 48.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). FZD4 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
SFRP2 Human
Secreted Frizzled-Related Protein 2 Human Recombinant
SFRP2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
SFRP2 Mouse
Secreted Frizzled-Related Protein 2 Mouse Recombinant
SFRP2 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 280 amino acids (25-295 a.a) and having a molecular mass of 32.1kDa.
SFRP2 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
SFRP4 Human, sf9
Secreted Frizzled-Related Protein 4 Human Recombinant, sf9
SFRP4 Human
Secreted Frizzled-Related Protein 4 Human Recombinant
The Human SFRP4 is purified by proprietary chromatographic techniques.
SFRP5 Human
Secreted Frizzled-Related Protein 5 Human Recombinant
SFRP5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Secreted Frizzled-Related Proteins (SFRPs) are a family of secreted glycoproteins that act as extracellular signaling ligands. They are structurally related to Frizzled (Fz) receptors, sharing a cysteine-rich domain (CRD) that enables them to bind to Wnt ligands or Fz receptors . The SFRP family consists of five members: SFRP1, SFRP2, SFRP3, SFRP4, and SFRP5 .
Key Biological Properties: SFRPs are known for their ability to bind Wnt proteins and Fz receptors, thereby modulating Wnt signaling pathways . They can act as antagonists to Wnt signaling by preventing Wnt proteins from binding to Fz receptors .
Expression Patterns and Tissue Distribution: SFRPs are expressed in various tissues and have distinct expression patterns. For example, SFRP1 is expressed in the retina, SFRP2 in the heart and vasculature, SFRP3 in the brain, SFRP4 in the pancreas, and SFRP5 in adipose tissue .
Primary Biological Functions: SFRPs play crucial roles in regulating cell growth, differentiation, and tissue homeostasis . They are involved in processes such as embryonic development, bone formation, and angiogenesis .
Role in Immune Responses and Pathogen Recognition: SFRPs have been implicated in modulating immune responses by influencing the activity of immune cells and cytokine production . They can also play a role in pathogen recognition by interacting with receptors on immune cells .
Mechanisms with Other Molecules and Cells: SFRPs interact with Wnt proteins and Fz receptors in the extracellular compartment, forming inhibitory complexes that prevent Wnt signaling . They can also bind to other receptors and matrix molecules, influencing various signaling pathways .
Binding Partners and Downstream Signaling Cascades: SFRPs bind to Wnt ligands and Fz receptors, modulating downstream signaling cascades such as the canonical Wnt/β-catenin pathway and noncanonical Wnt/Ca2+ pathway . These interactions can lead to changes in gene transcription and cell behavior .
Transcriptional Regulation: The expression of SFRPs is regulated at the transcriptional level by various transcription factors and signaling pathways . For example, Wnt signaling itself can regulate the expression of SFRPs through feedback mechanisms .
Post-Translational Modifications: SFRPs undergo post-translational modifications such as glycosylation, which can affect their stability, secretion, and interactions with other molecules .
Biomedical Research: SFRPs are used as tools to study Wnt signaling pathways and their roles in development and disease . They are also investigated as potential biomarkers for various diseases, including cancer and metabolic disorders .
Diagnostic Tools and Therapeutic Strategies: SFRPs have potential applications in diagnostics and therapeutics. For example, SFRP2 is being explored as a target for anti-angiogenic therapies in cancer . SFRP4 has been identified as a biomarker for β-cell dysfunction and insulin resistance in diabetes .
Development: SFRPs are essential for proper embryonic development, influencing processes such as cell differentiation, tissue patterning, and organogenesis .
Aging and Disease: The expression and activity of SFRPs can change with aging, contributing to age-related diseases such as cancer, osteoporosis, and neurodegenerative disorders . Dysregulation of SFRP expression is also associated with various pathologies, including cardiovascular diseases and metabolic disorders .
