FZD4 Human
Frizzled-4, Fz-4, hFz4, FzE4, CD344, FZD4.
Greater than 90.0% as determined by SDS-PAGE.
Description
FZD4 Human Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 428 amino acids (37-222 a.a.) and having a molecular mass of 48.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). FZD4 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Frizzled-4, Fz-4, hFz4, FzE4, CD344, FZD4.
ADPFGDEEER RCDPIRISMC QNLGYNVTKM PNLVGHELQT DAELQLTTFT PLIQYGCSSQ LQFFLCSVYV PMCTEKINIP IGPCGGMCLS VKRRCEPVLK EFGFAWPESL NCSKFPPQND HNHMCMEGPG DEEVPLPHKT PIQPGEECHS VGTNSDQYIW VKRSLNCVLK CGYDAGLYSR
SAKEFTDIWL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG
QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH
Product Science Overview
FZD4 is a seven-transmembrane glycoprotein that functions as a receptor for Wnt proteins . It can activate several signaling pathways, including the canonical Wnt/β-catenin pathway, planar cell polarity pathway, and calcium flux pathway . The activation of these pathways leads to various cellular responses, such as cell proliferation, differentiation, and migration.
FZD4 is particularly important in the development of the vascular system in the retina. It acts as a receptor for both Wnt proteins and Norrin, a protein that is crucial for retinal vascularization . Mutations in the FZD4 gene have been associated with familial exudative vitreoretinopathy (FEVR), a genetic disorder that affects the development of blood vessels in the retina .
Recombinant human FZD4 is produced using recombinant DNA technology, which involves inserting the human FZD4 gene into a suitable expression system, such as a bacterial or mammalian cell line. This allows for the production of large quantities of the protein for research and therapeutic purposes .
The recombinant protein is typically purified to high levels of purity and is used in various applications, including functional assays, structural studies, and drug development . It is often used in studies to understand the mechanisms of Wnt signaling and to develop potential therapies for diseases related to this pathway.
Recombinant human FZD4 is used in a variety of research applications, including:
- Functional Assays: To study the binding and activation of Wnt proteins and other ligands.
- Structural Studies: To determine the three-dimensional structure of the receptor and understand its interactions with ligands.
- Drug Development: To screen for potential therapeutic compounds that can modulate the activity of FZD4 and the Wnt signaling pathway.
