Recombinant Proteins
Product List
CHMP1B Human
Charged Multivesicular Body Protein 1B Human Recombinant
CHMP1B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CHMP5 Human
Charged Multivesicular Body Protein 5 Human Recombinant
CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CHMP6 Human
Charged Multivesicular Body Protein 6 Human Recombinant
CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Charged Multivesicular Body Proteins (CHMPs) are a family of proteins that are integral components of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. These proteins are involved in the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into intraluminal vesicles (ILVs). CHMPs are classified into several subtypes, including CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C, CHMP5, CHMP6, CHMP7, and CHMP8 .
Key Biological Properties: CHMPs are involved in membrane remodeling and the release of endosomal intraluminal vesicles into multivesicular bodies. They are essential for the degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes, and lipids .
Expression Patterns and Tissue Distribution: CHMPs are ubiquitously expressed in various tissues, with notable expression in neurons of all major regions of the brain . For example, CHMP2B is highly active in the brain and is essential for the survival of nerve cells (neurons) .
Primary Biological Functions: CHMPs play a crucial role in the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these bodies. They are involved in the degradation of membrane proteins and the recycling or degradation of cell surface receptors .
Role in Immune Responses and Pathogen Recognition: CHMPs are involved in the immune response by mediating the degradation of pathogen-associated molecular patterns (PAMPs) and other immune-related proteins. They also play a role in the presentation of antigens to the immune system .
Mechanisms with Other Molecules and Cells: CHMPs interact with various molecules and cells through the ESCRT-III complex. They bind to other ESCRT components and facilitate the invagination and scission of endosomal membranes .
Binding Partners and Downstream Signaling Cascades: CHMPs have several binding partners, including other ESCRT-III subunits and membrane lipids. They are involved in downstream signaling cascades that regulate membrane remodeling and protein sorting .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of CHMPs are regulated by various mechanisms, including transcriptional regulation and post-translational modifications. For example, the C-terminal domain of CHMP2B regulates its activity by keeping the protein inactive until it is needed .
Transcriptional Regulation and Post-Translational Modifications: CHMPs undergo various post-translational modifications, such as phosphorylation, which can affect their function and interactions with other proteins .
Biomedical Research: CHMPs are studied for their role in various cellular processes, including membrane trafficking, autophagy, and protein degradation. They are also investigated for their involvement in neurodegenerative diseases, such as frontotemporal dementia and amyotrophic lateral sclerosis .
Diagnostic Tools and Therapeutic Strategies: CHMPs have potential applications as diagnostic biomarkers and therapeutic targets. For example, CHMP2B mutations are associated with neurodegenerative diseases, making it a potential target for therapeutic interventions .
Role Throughout the Life Cycle: CHMPs play a role throughout the life cycle, from development to aging and disease. They are involved in various cellular processes, including endocytosis, autophagy, and protein degradation, which are essential for maintaining cellular homeostasis .
From Development to Aging and Disease: CHMPs are crucial for the proper functioning of cells during development and aging. Mutations or dysregulation of CHMPs can lead to various diseases, including neurodegenerative disorders and cancer .
