CHMP6 Human
Charged Multivesicular Body Protein 6 Human Recombinant
Cat. No.
BT7558
Source
Escherichia Coli.
Synonyms
Charged multivesicular body protein 6, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
CHMP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-201 a.a) and having a molecular mass of 26.1kDa (Molecular size on SDS-PAGE will appear higher).
CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CHMP6 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Charged multivesicular body protein 6 (CHMP6), a member of the SNF7 family, is crucial for the formation of multivesicular bodies (MVBs) and the trafficking of proteins within them. As a core component of the endosomal sorting complex required for transport III (ESCRT-III), CHMP6 participates in the creation of intraluminal vesicles (ILVs) within MVBs. These ILVs encapsulate various molecules destined for degradation in lysosomes, including activated growth factor receptors, lysosomal enzymes, and lipids.
Description
Recombinant human CHMP6, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 225 amino acids (residues 1-201). With a molecular weight of 26.1 kDa, it appears larger on SDS-PAGE due to the presence of a 24 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The CHMP6 protein solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 10% glycerol, and 100 mM NaCl.
Stability
For short-term storage (up to 4 weeks), the protein can be stored at 4°C. For extended periods, storage at -20°C is recommended. To ensure stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advisable. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the CHMP6 protein is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
Charged multivesicular body protein 6, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERA LARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQ VMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAI LEELSAITQE QIELPEVPSE PLPEKIPENV PVKARPRQAE LVAAS.
Product Science Overview
Gene and Protein Structure
Function and Mechanism
CHMP6 is involved in the formation of MVBs, which are endosomal structures containing intraluminal vesicles (ILVs). These ILVs are generated by the invagination and scission from the limiting membrane of the endosome. MVBs are primarily delivered to lysosomes, where they enable the degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes, and lipids .
The ESCRT-III complex, which includes CHMP6, is essential for various cellular processes, including:
- Degradation of surface receptors: CHMP6 helps in the downregulation of surface receptors by sorting them into MVBs for degradation.
- Cytokinesis: The ESCRT-III complex is involved in the final stages of cell division, ensuring proper separation of daughter cells.
- Viral budding: CHMP6 and other ESCRT-III proteins facilitate the budding of enveloped viruses, such as HIV-1, from the host cell membrane .
Clinical Significance
Mutations or dysregulation of CHMP6 have been associated with several diseases, including:
Research and Applications
Human recombinant CHMP6 is used in various research applications to study its role in the ESCRT-III complex and its involvement in cellular processes. Understanding the function and regulation of CHMP6 can provide insights into the mechanisms of diseases associated with its dysregulation and potential therapeutic targets.
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