CHMP1B Human

Charged Multivesicular Body Protein 1B Human Recombinant
Cat. No.
BT7430
Source
Escherichia Coli.
Synonyms
Charged multivesicular body protein 1b, CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2, CHMP1B, C18orf2, Vps46B, C10orf2, C18-ORF2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

CHMP1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 223 amino acids (1-199 a.a) and having a molecular mass of 24.7kDa (Molecular weight on SDS-PAGE will appear higher).
CHMP1B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) family, charged multivesicular body protein 1b (CHMP1B) may be a peripherally associated component of the ESCRT-III complex (endosomal sorting required for transport complex III). This complex plays a crucial role in the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these MVBs. MVBs are characterized by the presence of intraluminal vesicles (ILVs) formed through invagination and scission from the limiting membrane of the endosome. These ILVs are typically transported to lysosomes, facilitating the degradation of various molecules, including stimulated growth factor receptors, lysosomal enzymes, and lipids.
Description
Recombinant CHMP1B, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 223 amino acids (1-199 a.a) with a molecular mass of 24.7 kDa. Note that the molecular weight observed on SDS-PAGE may be higher. This protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The CHMP1B protein solution (0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 85.0% as assessed by SDS-PAGE.
Synonyms
Charged multivesicular body protein 1b, CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2, CHMP1B, C18orf2, Vps46B, C10orf2, C18-ORF2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV GTSVASAEQD ELSQRLARLR DQV.

Product Science Overview

Function and Role in Cellular Processes

CHMP1B is a component of the endosomal sorting complex required for transport III (ESCRT-III) . The ESCRT-III complex is crucial for the formation of multivesicular bodies (MVBs) and the sorting of endosomal cargo proteins into these MVBs . MVBs are specialized endosomes that contain intraluminal vesicles (ILVs) formed by the invagination and scission from the limiting membrane of the endosome . These ILVs are typically delivered to lysosomes, where they enable the degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes, and lipids .

The ESCRT-III complex also plays a role in other topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses like HIV-1 .

Genetic and Molecular Information

The CHMP1B gene is located on chromosome 18 in humans . It has several aliases, including C18orf2, Vps46B, and hVps46-2 . The protein encoded by this gene is involved in various cellular processes, including the regulation of centrosome duplication, nucleus organization, and the establishment of protein localization .

Clinical Significance

Mutations or dysregulation of the CHMP1B gene have been associated with certain diseases. For example, CHMP1B is linked to Pontocerebellar Hypoplasia, Type 7 and Deafness, Autosomal Recessive 20 . These associations highlight the importance of CHMP1B in normal cellular function and development.

Research and Applications

Recombinant CHMP1B protein is used in various research applications to study its function and role in cellular processes. Understanding the mechanisms by which CHMP1B and the ESCRT-III complex operate can provide insights into the regulation of endosomal sorting and the degradation of membrane proteins .

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