Recombinant Proteins
Product List
LBP Human
Lipopolysaccaride Human Recombinant
The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE.
Attention: His-tag has no protease site and can not be split off.
LBP Human, HEK
Lipopolysaccarid Binding Protein, Human Recombinant, HEK
LBP Human Recombinant is a single, glycosylated polypeptide chain containing 462 amino acids (26-481a.a) and having a molecular mass of 51.7kDa (calculated). LBP is fused to a 6 a.a His tag at C-terminal.
HEK293 Cells.
LBP Mouse
Lipopolysaccaride Mouse Recombinant
Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.
Introduction
Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that plays a crucial role in the immune response to bacterial infections. It binds to bacterial lipopolysaccharides (LPS), which are components of the outer membrane of Gram-negative bacteria, and presents them to cell surface receptors such as CD14 and TLR4 . LBP is classified as an acute-phase protein due to its rapid increase in concentration in response to inflammation.
Key Biological Properties: LBP is known for its ability to bind LPS and facilitate its recognition by the immune system. This binding is essential for the activation of immune responses against Gram-negative bacteria .
Expression Patterns: LBP is primarily expressed in the liver and is secreted into the bloodstream. It is also found in other tissues such as the lungs and intestines .
Tissue Distribution: LBP is predominantly found in the liver, but it is also present in other tissues including the lungs, intestines, and blood plasma .
Primary Biological Functions: The primary function of LBP is to bind LPS and present it to CD14 and TLR4 receptors on immune cells, thereby initiating an immune response . This process is critical for the detection and elimination of Gram-negative bacteria.
Role in Immune Responses: LBP plays a significant role in the innate immune response by recognizing and binding to LPS, which triggers the activation of immune cells and the production of inflammatory cytokines .
Pathogen Recognition: By binding to LPS, LBP helps the immune system recognize and respond to Gram-negative bacterial infections .
Mechanisms with Other Molecules and Cells: LBP interacts with CD14 and TLR4 receptors on the surface of immune cells. This interaction facilitates the transfer of LPS to these receptors, leading to the activation of downstream signaling pathways .
Binding Partners: The primary binding partners of LBP are LPS, CD14, and TLR4 .
Downstream Signaling Cascades: Upon binding to LPS, LBP facilitates the transfer of LPS to CD14 and TLR4, which activates downstream signaling pathways such as the NF-κB pathway. This results in the production of pro-inflammatory cytokines and the activation of immune responses .
Regulatory Mechanisms: The expression and activity of LBP are regulated at multiple levels, including transcriptional and post-translational modifications .
Transcriptional Regulation: The transcription of the LBP gene is upregulated in response to inflammatory signals such as cytokines (e.g., IL-6) and bacterial infections .
Post-Translational Modifications: LBP undergoes various post-translational modifications that can affect its stability, localization, and activity .
Biomedical Research: LBP is used as a biomarker for the detection of bacterial infections and sepsis .
Diagnostic Tools: LBP levels in the blood can be measured to diagnose and monitor bacterial infections and inflammatory conditions .
Therapeutic Strategies: Targeting LBP or its interactions with LPS and immune receptors is being explored as a potential therapeutic strategy for treating sepsis and other inflammatory diseases .
Role Throughout the Life Cycle: LBP plays a critical role in the immune system throughout the life cycle. It is involved in the early detection and response to bacterial infections, which is essential for maintaining health and preventing disease .
From Development to Aging and Disease: The expression and activity of LBP can be influenced by various factors throughout life, including developmental stages, aging, and the presence of diseases such as infections and chronic inflammatory conditions .
