Recombinant Proteins
Product List
FBXO2 Human
F-Box Protein 2 Human Recombinant
FBXO2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
FBXO6 Human
F-Box Protein 6 Human Recombinant
FBXO6 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Introduction
F-box proteins are a class of proteins characterized by the presence of an F-box motif, a protein structural motif of approximately 50 amino acids that mediates protein-protein interactions . These proteins were first identified as components of the SCF (Skp1-Cullin-F-box) ubiquitin-ligase complex, which plays a crucial role in ubiquitin-mediated proteolysis . F-box proteins are classified based on the presence of additional motifs, such as leucine-rich repeats (LRRs) and WD repeats, leading to three main categories: FBXL (F-box and LRRs), FBXW (F-box and WD repeats), and FBXO (F-box with other or no additional motifs) .
F-box proteins exhibit diverse biological properties and are widely expressed across various tissues. They are pivotal in regulating cellular processes through their role in the SCF complex, which tags proteins for degradation by the 26S proteasome . The expression patterns of F-box proteins can vary significantly, with some being ubiquitously expressed while others are tissue-specific . For instance, in plants, F-box proteins are involved in processes such as seed germination and response to abiotic stress .
The primary biological function of F-box proteins is to serve as substrate adaptors in the SCF complex, facilitating the ubiquitination and subsequent degradation of target proteins . This function is critical for various cellular processes, including cell cycle regulation, signal transduction, and response to environmental stimuli . In the immune system, F-box proteins play roles in pathogen recognition and immune responses by regulating the stability of key signaling molecules .
F-box proteins interact with other molecules and cells primarily through their role in the SCF complex. They bind to the core SCF component Skp1 via the F-box motif and to specific substrates through additional protein-protein interaction motifs . This interaction positions the substrate for ubiquitination by the SCF complex, leading to its degradation . The downstream signaling cascades activated by this process are essential for maintaining cellular homeostasis and responding to external signals .
The expression and activity of F-box proteins are tightly regulated through various mechanisms. Transcriptional regulation ensures that F-box proteins are produced in response to specific cellular needs . Post-translational modifications, such as phosphorylation, ubiquitination, and glycosylation, further modulate their activity and stability . For example, the F-box protein Met30 in yeast can be ubiquitinated in a cullin-dependent manner, affecting its function .
F-box proteins have significant applications in biomedical research, diagnostics, and therapeutic strategies. Their role in protein degradation makes them potential targets for drug development, particularly in cancer therapy where dysregulation of protein degradation pathways is common . Additionally, F-box proteins can be used as biomarkers for certain diseases, aiding in early diagnosis and treatment .
Throughout the life cycle, F-box proteins are involved in various stages of development, aging, and disease. During embryogenesis, they regulate the degradation of proteins essential for cell division and differentiation . In adulthood, they continue to maintain cellular homeostasis and respond to environmental changes . Dysregulation of F-box proteins has been linked to age-related diseases and cancer, highlighting their importance in maintaining cellular function throughout life .
