Recombinant Proteins
Product List
DCTN6 Human
Dynactin 6 Human Recombinant
DCTN2 (1-401) Human
Dynactin 2 (1-401 a.a.) Human Recombinant
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCTN2 (1-403) Human
Dynactin 2 (1-403 a.a.) Human Recombinant
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCTN2 (1-406) Human
Dynactin 2 (1-406 a.a.) Human Recombinant
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Dynactin is a multi-subunit protein complex that acts as a co-factor for the microtubule motor protein cytoplasmic dynein-1. It is composed of 23 subunits and is built around a short filament of actin-related protein-1 (Arp1) . Dynactin is essential for the activation and processivity of dynein, facilitating the transport of various cellular cargos along microtubules .
Key Biological Properties: Dynactin is crucial for dynein activity and can be thought of as a “dynein receptor” that modulates the binding of dynein to cell organelles . It enhances the processivity of cytoplasmic dynein and kinesin-2 motors .
Expression Patterns and Tissue Distribution: Dynactin is ubiquitously expressed in eukaryotic cells, with its components being essential for various cellular processes. The expression of dynactin subunits is tightly regulated to ensure proper cellular function .
Primary Biological Functions: Dynactin plays a pivotal role in intracellular transport by facilitating the movement of organelles, vesicles, and other cargos along microtubules . It is involved in processes such as mitosis, endocytosis, and organelle positioning .
Role in Immune Responses and Pathogen Recognition: While dynactin’s primary role is in intracellular transport, it indirectly supports immune responses by ensuring the proper distribution and function of immune-related organelles and vesicles .
Mechanisms with Other Molecules and Cells: Dynactin interacts with dynein and various adaptor proteins to form a highly processive motor complex . It binds to microtubules and cargos through its subunits, facilitating the transport process .
Binding Partners and Downstream Signaling Cascades: Dynactin binds to dynein through its p150Glued subunit and interacts with other proteins such as BICD2 and Hook1, which act as activating adaptors . These interactions enhance the stability and processivity of the dynein-dynactin complex .
Regulatory Mechanisms Controlling Expression and Activity: The expression of dynactin subunits is regulated at the transcriptional level by various transcription factors . Post-translational modifications, such as phosphorylation, also play a role in modulating dynactin activity .
Transcriptional Regulation and Post-Translational Modifications: Specific transcription factors regulate the expression of dynactin subunits, ensuring their proper levels in the cell . Post-translational modifications, including phosphorylation and ubiquitination, can alter the activity and stability of dynactin .
Biomedical Research: Dynactin is a critical component in studies of intracellular transport and motor protein function. It is used to understand the mechanisms of dynein-mediated transport and its role in various cellular processes .
Diagnostic Tools and Therapeutic Strategies: Dysregulation of dynactin has been implicated in neurodegenerative diseases and cancer. Understanding its function and regulation can lead to the development of diagnostic markers and therapeutic targets .
Role Throughout the Life Cycle: Dynactin is essential for various stages of the cell cycle, including mitosis and cytokinesis . It ensures proper chromosome segregation and spindle positioning during cell division . Dysregulation of dynactin can lead to developmental abnormalities and diseases .
From Development to Aging and Disease: Dynactin plays a crucial role in development by facilitating the proper distribution of cellular components. Its function is also vital in maintaining cellular homeostasis during aging . Dysregulation of dynactin has been linked to neurodegenerative diseases, highlighting its importance in disease pathology .
