DCTN2 (1-406) Human
Dynactin 2 (1-406 a.a.) Human Recombinant
Cat. No.
BT30868
Source
Escherichia Coli.
Synonyms
DCTN2, Dynactin 2 (P50), DCTN50, Dynactin Complex 50 KDa Subunit, 50 KDa Dynein-Associated Polypeptide, P50 Dynamitin, 50 KD Dynein-Associated Polypeptide, DYNAMITIN, HEL-S-77, RBP50, Dynactin Complex 50 KD Subunit, Dynactin Subunit 2, Epididymis Secretory Protein Li 77, DCTN-50.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Dynactin 2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 429 amino acids (1-406 a.a) and having a molecular mass of 47.2kDa.
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
DCTN2, a 50 kDa subunit of the dynactin complex, plays a crucial role in various cellular processes. Dynactin, comprising 10-11 subunits (22-150 kDa), interacts with microtubules and cytoplasmic dynein to regulate functions such as ER-to-Golgi transport, lysosome and endosome movement, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. Present in 4-5 copies per dynactin molecule, DCTN2, with its three short alpha-helical coiled-coil domains, facilitates interactions with other dynactin subunits, particularly the largest subunit (p150). This interaction is vital for stabilizing p150 and modulating cytoplasmic dynein's binding to organelles. DCTN2 also contributes to chromosome alignment during prometaphase and spindle organization during mitosis, playing a crucial role in anchoring microtubules to centrosomes and synapse formation during brain development.
Description
Recombinant Human Dynactin 2, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 429 amino acids (1-406 a.a) with a molecular weight of 47.2 kDa. This protein is purified using proprietary chromatographic techniques and includes a 23 amino acid His-tag at the N-terminus.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The DCTN2 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and contains additional components such as 20% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For optimal storage and product longevity, it is recommended to store the unopened vial at 4°C for short-term use (2-4 weeks). For extended storage, freezing at -20°C is advised. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is suggested. It is crucial to minimize repeated freeze-thaw cycles to preserve protein integrity and activity.
Purity
The purity of this protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
DCTN2, Dynactin 2 (P50), DCTN50, Dynactin Complex 50 KDa Subunit, 50 KDa Dynein-Associated Polypeptide, P50 Dynamitin, 50 KD Dynein-Associated Polypeptide, DYNAMITIN, HEL-S-77, RBP50, Dynactin Complex 50 KD Subunit, Dynactin Subunit 2, Epididymis Secretory Protein Li 77, DCTN-50.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAFA QELEELTSTS VEHIIVNPNA AYDKFKDKRV GTKGLDFSDR IGKTKRTGYE SGEYEMLGEG LGVKETPQQK YQRLLHEVQE LTTEVEKIKT TVKESATEEK LTPVLLAKQL AALKQQLVAS HLEKLLGPDA AINLTDPDGA LAKRLLLQLE ATKNSKGGSG GKTTGTPPDS SLVTYELHSR PEQDKFSQAA KVAELEKRLT ELETAVRCDQ DAQNPLSAGL QGACLMETVE LLQAKVSALD LAVLDQVEAR LQSVLGKVNE IAKHKASVED ADTQSKVHQL YETIQRWSPI ASTLPELVQR LVTIKQLHEQ AMQFGQLLTH LDTTQQMIAN SLKDNTTLLT QVQTTMRENL ATVEGNFASI DERMKKLGK.
Product Science Overview
Introduction
Dynactin 2, also known as dynamitin or DCTN2, is a crucial subunit of the dynactin complex, a multi-subunit protein complex that plays a significant role in cellular processes involving microtubule-based transport. The recombinant form of Dynactin 2 (1-406 amino acids) is produced using recombinant DNA technology, which allows for the expression of this protein in a controlled environment, typically in bacterial systems like E. coli.
Structure and Composition
Dynactin 2 is a 50-kilodalton (kDa) subunit of the dynactin complex, which consists of 23 subunits in total. The recombinant form of Dynactin 2 (1-406 a.a.) is a non-glycosylated polypeptide chain containing 429 amino acids, including a 23 amino acid His-tag at the N-terminus for purification purposes . The His-tag facilitates the purification of the protein using affinity chromatography techniques.
Function
Dynactin 2 is integral to the dynactin complex, which acts as a co-factor for the microtubule motor protein cytoplasmic dynein-1. This complex is essential for various cellular functions, including:
- Vesicle Transport: Dynactin 2 aids in the movement of membrane vesicles along microtubules, facilitating intracellular transport processes .
- Spindle Formation and Chromosome Movement: It plays a role in mitotic spindle formation and the movement of chromosomes during cell division .
- Nuclear Positioning and Axonogenesis: Dynactin 2 is involved in the positioning of the nucleus and the formation of axons in neurons .
Production of Recombinant Dynactin 2
The production of recombinant Dynactin 2 involves several steps:
- Gene Cloning: The gene encoding Dynactin 2 is cloned into an expression vector, which is then introduced into a host organism, typically E. coli .
- Protein Expression: The host cells are cultured under conditions that induce the expression of the recombinant protein .
- Purification: The His-tagged Dynactin 2 is purified using affinity chromatography, which exploits the affinity of the His-tag for nickel ions .
Applications
Recombinant Dynactin 2 is used in various research applications, including:
- Cell Biology Studies: It is used to study the role of the dynactin complex in cellular processes such as vesicle transport and mitosis .
- Neuroscience Research: Researchers use it to investigate the mechanisms of axonogenesis and synapse formation .
- Drug Development: Dynactin 2 can be used in screening assays to identify compounds that affect microtubule-based transport processes .
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