DCTN2 (1-401) Human

Dynactin 2 (1-401 a.a.) Human Recombinant
Cat. No.
BT30818
Source
Escherichia Coli.
Synonyms
DCTN2, Dynactin 2 (P50), DCTN50, Dynactin Complex 50 KDa Subunit, 50 KDa Dynein-Associated Polypeptide, P50 Dynamitin, 50 KD Dynein-Associated Polypeptide, DYNAMITIN, HEL-S-77, RBP50, Dynactin Complex 50 KD Subunit, Dynactin Subunit 2, Epididymis Secretory Protein Li 77, DCTN-50.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Dynactin 2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-401 a.a) and having a molecular mass of 46.6kDa.
DCTN2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
DCTN2, a 50kDa protein, is a subunit of the dynactin complex. Dynactin, a multi-subunit complex ranging from 22 to 150 kDa, interacts with both microtubules and cytoplasmic dynein. This interaction is crucial for various cellular processes, including ER-to-Golgi transport, lysosome and endosome movement, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. A dynactin molecule typically contains 4-5 copies of the DCTN2 subunit. Structurally, DCTN2 comprises three short alpha-helical coiled-coil domains, facilitating interactions with itself and other dynactin subunits. Notably, DCTN2 directly interacts with the p150 subunit, the largest in the dynactin complex, effectively anchoring it. DCTN2 also regulates the binding of cytoplasmic dynein to organelles and participates in prometaphase chromosome alignment and spindle organization during mitosis. Furthermore, DCTN2 contributes to anchoring microtubules to centrosomes and plays a role in synapse formation during brain development.
Description
Recombinant Human Dynactin 2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 424 amino acids, with a sequence spanning from amino acid 1 to 401, and possesses a molecular weight of 46.6kDa. The DCTN2 protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The DCTN2 protein solution is provided at a concentration of 0.5mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 4 weeks), the DCTN2 protein solution should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. It's important to minimize repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the DCTN2 protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
DCTN2, Dynactin 2 (P50), DCTN50, Dynactin Complex 50 KDa Subunit, 50 KDa Dynein-Associated Polypeptide, P50 Dynamitin, 50 KD Dynein-Associated Polypeptide, DYNAMITIN, HEL-S-77, RBP50, Dynactin Complex 50 KD Subunit, Dynactin Subunit 2, Epididymis Secretory Protein Li 77, DCTN-50.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADPKYA DLPGIARNEP DVYETSDLPE DDQAEFDAEE LTSTSVEHII VNPNAAYDKF KDKRVGTKGL DFSDRIGKTK RTGYESGEYE MLGEGLGVKE TPQQKYQRLL HEVQELTTEV EKIKTTVKES ATEEKLTPVL LAKQLAALKQ QLVASHLEKL LGPDAAINLT DPDGALAKRL LLQLEATKNS KGGSGGKTTG TPPDSSLVTY ELHSRPEQDK FSQAAKVAEL EKRLTELETA VRCDQDAQNP LSAGLQGACL METVELLQAK VSALDLAVLD QVEARLQSVL GKVNEIAKHK ASVEDADTQS KVHQLYETIQ RWSPIASTLP ELVQRLVTIK QLHEQAMQFG QLLTHLDTTQ QMIANSLKDN TTLLTQVQTT MRENLATVEG NFASIDERMK KLGK.

Product Science Overview

Introduction

Dynactin 2, also known as DCTN2, is a crucial component of the dynactin complex, which is essential for various cellular processes, including intracellular transport, mitosis, and maintenance of cell structure. The recombinant form of Dynactin 2 (1-401 a.a.) is a human protein expressed in E. coli, consisting of the first 401 amino acids of the full-length protein.

Structure and Expression

The recombinant Dynactin 2 (1-401 a.a.) is a single, non-glycosylated polypeptide chain containing 424 amino acids, including a 23 amino acid His-tag at the N-terminus. This His-tag facilitates purification through affinity chromatography . The molecular weight of this recombinant protein is approximately 46.6 kDa .

Function and Role in Cellular Processes

Dynactin 2 plays a pivotal role in the dynactin complex, which is involved in the regulation of dynein motor activity. Dynein is a motor protein that moves along microtubules, transporting various cellular cargoes, including organelles, vesicles, and proteins. The dynactin complex enhances dynein’s processivity and cargo-binding capabilities, making it essential for efficient intracellular transport.

In addition to its role in transport, Dynactin 2 is involved in mitosis, where it helps in the proper alignment and segregation of chromosomes. It also contributes to the maintenance of cell structure by stabilizing microtubules and ensuring proper cell morphology.

Applications in Research

Recombinant Dynactin 2 (1-401 a.a.) is widely used in research to study the functions and mechanisms of the dynactin complex. It serves as a valuable tool for investigating the interactions between dynactin and dynein, as well as other cellular components involved in intracellular transport and mitosis. Researchers utilize this recombinant protein to elucidate the molecular basis of various diseases associated with defects in the dynactin-dynein pathway, such as neurodegenerative disorders and cancer.

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