Recombinant Proteins
Product List
ASF1A Human
ASF1 Anti-Silencing Function 1 Homolog A Human Recombinant
ASF1A is fused to a 36 amino acid His Tag and purified by proprietary chromatographic techniques.
ASF1B Human
ASF1 Anti-Silencing Function 1 Homolog B Human Recombinant
ASF1B is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Introduction
Anti-Silencing Function 1 (ASF1) is a highly conserved histone H3/H4 chaperone protein involved in various chromatin-related processes, including nucleosome assembly and disassembly, DNA replication, transcription, and DNA repair . ASF1 exists in two paralogs, ASF1A and ASF1B, which are found in a wide range of eukaryotic organisms .
Key Biological Properties: ASF1 is crucial for S-phase progression and cell proliferation . It interacts with histones H3 and H4 to facilitate their deposition and removal from chromatin .
Expression Patterns and Tissue Distribution: ASF1A and ASF1B are expressed in proliferative tissues and are regulated during cell cycle progression . In Arabidopsis, ASF1A and ASF1B are mainly expressed in proliferative tissues and are associated with the UV-B-induced DNA damage response .
Primary Biological Functions: ASF1 plays a significant role in chromatin assembly and disassembly, DNA replication, transcription, and DNA repair . It is involved in the deposition and removal of histone H3 and H4 during these processes .
Role in Immune Responses and Pathogen Recognition: ASF1 has been implicated in the regulation of immune responses and pathogen recognition through its role in chromatin remodeling and gene expression .
Mechanisms with Other Molecules and Cells: ASF1 interacts with various histone chaperones, such as CAF-1 and HIRA, to facilitate nucleosome assembly . It also interacts with acetyltransferases of the Histone Acetyl Transferase subfamily, which are involved in cell cycle control and DNA repair .
Binding Partners and Downstream Signaling Cascades: ASF1 forms complexes with histones H3 and H4, and its interaction with these histones is essential for its function in chromatin assembly . ASF1 is also involved in the regulation of the PI3K/AKT signaling pathway in certain fungi .
Transcriptional Regulation: ASF1A and ASF1B are regulated by E2F transcription factors during cell cycle progression . Their expression is increased following UV-B treatment, indicating a role in the DNA damage response .
Post-Translational Modifications: ASF1 undergoes various post-translational modifications, including acetylation, which are crucial for its function in chromatin assembly and DNA repair .
Biomedical Research: ASF1 is used as a proliferation marker for cancer prognosis and is being studied for its role in tumor biology . It is also a promising prognostic biomarker and correlates with immunotherapy efficacy in hepatocellular carcinoma .
Diagnostic Tools and Therapeutic Strategies: ASF1’s role in DNA repair and cell cycle regulation makes it a potential target for therapeutic strategies aimed at enhancing DNA repair mechanisms in cancer treatment .
Development to Aging and Disease: ASF1 is essential for pre-implantation embryonic development and plays distinct roles during fertilization and early embryogenesis . It is also involved in the regulation of asexual and sexual reproduction in certain fungi . ASF1’s function in chromatin assembly and DNA repair is crucial throughout the life cycle, from development to aging and disease .
