ASF1B Human

ASF1B, or Anti-Silencing Function 1 Homolog B, is a member of the H3/H4 family of histone chaperone proteins. It is similar to the anti-silencing function-1 protein found in yeast. This protein plays a crucial role in the regulation of chromatin structure and function, which is essential for various cellular processes such as DNA replication, repair, and transcription.

The ASF1B gene is a protein-coding gene that is involved in chromatin regulation and acetylation pathways . The gene is located on chromosome 19 and is known to produce a histone chaperone protein that facilitates histone deposition, exchange, and removal during nucleosome assembly and disassembly . The ASF1B protein is also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) .

ASF1B cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly . It specifically recognizes and binds newly synthesized histones with the monomethylation of H3 ‘Lys-9’ (H3K9me1) and diacetylation at ‘Lys-5’ and ‘Lys-12’ of H4 (H4K5K12ac) marks in the cytosol . Unlike ASF1A, ASF1B does not participate in replication-independent nucleosome deposition .

ASF1B is required for gonad development and has been associated with various diseases, including Hermansky-Pudlak Syndrome 9 and Spastic Paraplegia 7, Autosomal Recessive . The protein’s role in ensuring a constant supply of histones at sites of nucleosome assembly is critical for maintaining chromatin structure and function .

ASF1B antibodies are used in various research applications, including Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), and Neutralization (Neut) . These antibodies are essential tools for studying the protein’s function and its role in chromatin regulation.