ASF1B Antibody
ASF1 Anti-Silencing Function 1 Homolog B, Mouse Anti Human
Cat. No.
BT12531
Source
Synonyms
Histone
chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b,
CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
ASF1 Anti-Silencing Function 1 Homolog B (ASF1B) is a histone chaperone protein belonging to the H3/H4 family. It shares similarities with the yeast anti-silencing function-1 protein. ASF1B serves as a substrate for cell cycle-regulated kinases of the tousled-like kinase family. It is believed to play a vital role in regulating chromatin's nucleosome structure by ensuring a consistent supply of histones at nucleosome assembly sites. Additionally, ASF1B collaborates with CAF-1 (chromatin assembly factor 1) to promote replication-dependent chromatin assembly. ASF1B exhibits high expression levels in the testis, with lower levels detected in the colon, small intestine, and thymus. Spermatogenesis relies on the presence of ASF1B.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The solution contains 1mg/ml of the antibody in a buffer of PBS at pH 7.4, supplemented with 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For storage up to 1 month, maintain the antibody at 4°C. For extended storage periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone testing using ELISA and Western blot analysis to confirm its specificity and reactivity. Optimal results may vary depending on the specific application. It is recommended to titrate the reagent for each investigation. A starting dilution of 1:1000 is suggested.
Synonyms
Histone
chaperone ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, hAsf1b,
CCG1-interacting factor A-II, CIA-II, hCIA-II, ASF1B.
Purification Method
ASF1B antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1D4AT.
Immunogen
Anti-human ASF1B mAb, clone PAT1D4AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ASF1B protein (1-202 amino acids) purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.
Product Science Overview
Structure and Function
ASF1B is the substrate of the tousled-like kinase family of cell cycle-regulated kinases. It cooperates with Chromatin Assembly Factor 1 (CAF-1) to stimulate replication-dependent chromatin assembly . This cooperation is essential for the proper assembly and disassembly of nucleosomes, which are the fundamental units of chromatin structure.
Expression and Localization
Immunogen and Antibody Production
The anti-human ASF1B monoclonal antibody, clone PAT1D4AT, is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ASF1B protein (1-202 amino acids) purified from E. coli . The antibody is of the IgG 2b subclass with a kappa light chain .
Applications
Storage and Stability
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