Recombinant Proteins
Product List
DCUN1D1 Human
DCN1 Defective in Cullin Neddylation 1 Domain Containing 1 Human Recombinant
DCUN1D1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCUN1D2 Human
DCN1 Defective in Cullin Neddylation 1 Domain Containing 2 Human Recombinant
DCUN1D2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 279 amino acids (1-259 a.a.) and having a molecular mass of 32.3kDa.
DCUN1D2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
DCUN1D4 Human
DCN1 Defective in Cullin Neddylation 1 Domain Containing 4 Human Recombinant
DCUN1D4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
DCUN1D (Defective in Cullin Neddylation 1 Domain Containing) proteins are a family of proteins that play a crucial role in the neddylation process, a post-translational modification similar to ubiquitination. This family includes several members, such as DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4, and DCUN1D5. These proteins are also known as squamous cell carcinoma-related oncogenes (SCCRO) due to their association with various cancers .
Key Biological Properties: DCUN1D proteins are involved in the neddylation of cullin proteins, which are components of E3 ubiquitin ligase complexes. This modification is essential for the activation of these complexes .
Expression Patterns and Tissue Distribution: DCUN1D proteins are expressed in various tissues, including lymphoid tissue, bone marrow, testis, skeletal muscle, and immune cells such as NK-cells and T-cells . Their expression levels can vary significantly depending on the tissue type and the presence of certain diseases, particularly cancers .
Primary Biological Functions: The primary function of DCUN1D proteins is to promote the neddylation of cullin proteins, thereby activating cullin-RING ubiquitin ligase complexes. These complexes are involved in various cellular processes, including cell cycle regulation, signal transduction, and protein degradation .
Role in Immune Responses and Pathogen Recognition: DCUN1D proteins, particularly DCUN1D1, have been shown to play a role in the innate immune response. They are involved in the regulation of immune cell proliferation and the response to stimuli .
Mechanisms with Other Molecules and Cells: DCUN1D proteins interact with several other proteins, including CUL3, CUL4B, RBX1, CAND1, and RPS19 . These interactions are crucial for their role in the neddylation process.
Binding Partners and Downstream Signaling Cascades: DCUN1D proteins act as scaffold-like E3 ligases, promoting the neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes. This modification activates these complexes, leading to the ubiquitination and subsequent degradation of target proteins .
Transcriptional Regulation: The expression of DCUN1D proteins is regulated at the transcriptional level by various transcription factors. For example, DCUN1D5 is a transcriptional target of the transcription factor Yin Yang 1 (YY1), which promotes its expression in triple-negative breast cancer .
Post-Translational Modifications: DCUN1D proteins themselves undergo post-translational modifications, such as phosphorylation, which can regulate their activity and stability .
Biomedical Research: DCUN1D proteins are of significant interest in cancer research due to their role in tumorigenesis. They are potential biomarkers for cancer diagnosis and prognosis .
Diagnostic Tools: The expression levels of DCUN1D proteins can be used as diagnostic markers for certain cancers, such as prostate cancer and triple-negative breast cancer .
Therapeutic Strategies: Targeting DCUN1D proteins with specific inhibitors could be a potential therapeutic strategy for cancers where these proteins are overexpressed .
Development to Aging and Disease: DCUN1D proteins play a role throughout the life cycle, from development to aging. Their dysregulation is associated with various diseases, particularly cancers. For example, DCUN1D1 is upregulated in prostate cancer and is essential for tumor growth and proliferation .
