Recombinant Proteins
Product List
LIN28 Human
LIN28 Human Recombinant
LIN28 is expressed with a 23 amino acid His tag fused at N-Terminus and purified by proprietary chromatographic techniques.
LIN28 Human, TAT
LIN28-TAT Human Recombinant
Recombinant Human LIN28 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 222 amino acids (including 13- residue C-terminal TAT peptide) and having a molecular mass of 24.4kDa.
The LIN28 is purified by proprietary chromatographic techniques.
LIN7A Human
LIN7A Human Recombinant
LIN7A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LIN7B Human
LIN7B Human Recombinant
LIN7B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LIN7C Human
LIN7C Human Recombinant
Introduction
LIN proteins, particularly LIN28, are RNA-binding proteins that play crucial roles in various biological processes. They are evolutionarily conserved and are known for their involvement in regulating developmental timing, pluripotency, and oncogenesis . LIN28 proteins are classified based on their RNA-binding domains, which include a cold-shock domain (CSD) and a zinc-knuckle domain (ZKD) .
Key Biological Properties: LIN28 proteins are best known for their role in promoting pluripotency by regulating the microRNA let-7 . They are highly expressed in embryonic stem cells and undifferentiated tissues and are downregulated as development and cellular differentiation proceed .
Expression Patterns: LIN28 is expressed early during development in various species, including humans, mice, C. elegans, Xenopus, zebrafish, and Drosophila . It is also highly expressed in embryonic stem cells and is downregulated in response to differentiation .
Tissue Distribution: LIN28 is predominantly found in undifferentiated tissues and embryonic stem cells. It is also expressed in certain cancers, where its expression is associated with less differentiated, more aggressive tumors .
Primary Biological Functions: LIN28 proteins are crucial for maintaining pluripotency and regulating the timing of developmental events . They play a significant role in the reprogramming of mammalian somatic cells to pluripotent cells .
Role in Immune Responses and Pathogen Recognition: While LIN28’s primary functions are related to development and pluripotency, its role in immune responses and pathogen recognition is not well-documented. However, its involvement in regulating cellular differentiation and proliferation may indirectly influence immune functions .
Mechanisms with Other Molecules and Cells: LIN28 proteins interact with microRNAs, particularly the let-7 family, to regulate their biogenesis . They bind to the let-7 pre-microRNA and block the production of mature let-7 microRNA .
Binding Partners and Downstream Signaling Cascades: LIN28 binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA . It also interacts with other mRNAs to modulate their translation and splicing events .
Transcriptional Regulation: LIN28 expression is tightly regulated during development. It is highly expressed in early development and downregulated as differentiation proceeds .
Post-Translational Modifications: LIN28 activity is regulated by various post-translational modifications, including phosphorylation and ubiquitination, which influence its stability and interaction with other molecules .
Biomedical Research: LIN28 is widely studied for its role in stem cell biology and cancer research. It is a key factor in the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) .
Diagnostic Tools: LIN28 expression levels can serve as biomarkers for certain cancers, indicating tumor aggressiveness and differentiation status .
Therapeutic Strategies: Targeting the LIN28/let-7 pathway holds potential for cancer therapy, as it regulates multiple oncogenic proteins .
Development: LIN28 is essential for early embryonic development and maintaining pluripotency in stem cells .
Aging and Disease: Aberrant expression of LIN28 is associated with various cancers and metabolic disorders. It can influence glucose homeostasis and resistance to obesity and type 2 diabetes .
