LIN28 Human
Greater than 85.0% as determined by SDS-PAGE
Description
LIN28 is expressed with a 23 amino acid His tag fused at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
LIN28 was first identified in the nematode Caenorhabditis elegans as a heterochronic gene that affects the timing of developmental events . In humans, there are two paralogs, LIN28A and LIN28B, which share similar functions but have distinct expression patterns and regulatory mechanisms .
The protein consists of two main RNA-binding domains:
LIN28 is best known for its role in regulating the biogenesis of the let-7 family of miRNAs. It binds to the terminal loop of pri- and pre-let-7 miRNAs, preventing their processing by Drosha and Dicer, which are enzymes involved in miRNA maturation . This inhibition leads to the suppression of let-7 miRNA levels, which in turn affects the expression of various target genes involved in cell proliferation and differentiation.
In addition to its role in miRNA regulation, LIN28 also binds to and modulates the translation of specific mRNAs. This dual function allows LIN28 to coordinate complex gene expression programs that are essential for maintaining stem cell pluripotency and promoting cellular reprogramming .
Aberrant expression of LIN28 has been linked to various human diseases, including cancer. Both LIN28A and LIN28B are overexpressed in several types of tumors, such as T-cell lymphoma, neuroblastoma, breast cancer, and hepatoblastoma . The oncogenic potential of LIN28 is primarily attributed to its ability to inhibit let-7 miRNA biogenesis, leading to the upregulation of oncogenes that are normally suppressed by let-7.
Recombinant LIN28 proteins are produced using genetic engineering techniques, where the LIN28 gene is cloned into an expression vector and introduced into a host cell system, such as bacteria or yeast. The host cells then produce the LIN28 protein, which can be purified and used for various research applications. Recombinant LIN28 is valuable for studying the protein’s structure, function, and interactions with other molecules, as well as for developing potential therapeutic strategies targeting LIN28-related pathways.
