LIN28 Human, TAT
Greater than 90% as determined by SDS-PAGE (coomassie staining).
Description
Recombinant Human LIN28 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 222 amino acids (including 13- residue C-terminal TAT peptide) and having a molecular mass of 24.4kDa.
The LIN28 is purified by proprietary chromatographic techniques.
Product Specs
Serving as a marker of undifferentiated human embryonic stem cells, LIN28 enhances the efficiency of induced pluripotent stem cell generation from human fibroblasts. By functioning as a 'translational enhancer,' LIN28 directs specific mRNAs to polysomes, resulting in elevated protein synthesis. In mouse embryonic stem cells, LIN28 binds to let-7 pre-miRNA, inhibiting the production of mature let-7 microRNA.
Recombinant Human LIN28, expressed in E.Coli, is a single, non-glycosylated polypeptide chain composed of 222 amino acids. This includes a 13-residue C-terminal TAT peptide, contributing to a molecular weight of 24.4kDa.
Purification of LIN28 is achieved using proprietary chromatographic methods.
The LIN28 protein solution is prepared in a buffer of PBS containing 50mM arginine.
SDS-PAGE analysis, using coomassie staining, indicates a purity exceeding 90%.
GPSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GPSAQGKPTY FREEEEEIHS PTLLPEAQNG GYGRKKRRQR RR.
Product Science Overview
LIN28 is an RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. It is abundantly found in embryonic stem cells (ESCs) and to a lesser extent in the placenta and testis . LIN28 plays a crucial role in blocking the processing and maturation of let-7 microRNA, which is a necessary step in the differentiation of stem cells and certain cancer cell lines .
LIN28, along with other regulatory proteins such as Sox2, Oct4, and Nanog, can induce the reprogramming of primary human fibroblasts to a pluripotent state . This reprogramming is essential for generating induced pluripotent stem cells (iPSCs), which have significant potential in regenerative medicine and research.
LIN28 and other regulatory proteins can be introduced into cells through various methods, including DNA transfection, viral infection, or microinjection . Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells .
The LIN28-TAT fusion protein is used in various research applications, including:
- Stem Cell Research: To study the role of LIN28 in stem cell maintenance and differentiation.
- Cancer Research: To investigate the involvement of LIN28 in cancer cell proliferation and differentiation.
- Regenerative Medicine: To explore the potential of LIN28 in reprogramming somatic cells to pluripotent stem cells.
