LIN7C Human

The recombinant human LIN7C protein is typically produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-197 a.a.) . It has a molecular mass of approximately 24.2 kDa and is often fused to a 23 amino acid His-tag at the N-terminus for purification purposes . The amino acid sequence of LIN7C includes several key regions that are essential for its function in protein-protein interactions and membrane association .

LIN7C is involved in the formation of multiprotein complexes that are essential for the polarized distribution of membrane proteins. These complexes play a significant role in various cellular processes, including signal transduction, cell adhesion, and intracellular trafficking . By regulating the localization and recycling of channels and receptors, LIN7C ensures that these proteins are correctly positioned at the plasma membrane, which is vital for the proper functioning of polarized cells .

Recombinant human LIN7C protein is widely used in research to study its role in cellular processes and its interactions with other proteins. It is particularly useful in experiments involving protein-protein interactions, membrane dynamics, and the mechanisms underlying cell polarity . The availability of recombinant LIN7C allows researchers to investigate its function in various model systems and to explore its potential implications in health and disease.

For optimal stability, recombinant human LIN7C protein should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein at -20°C and to avoid multiple freeze-thaw cycles . The protein is typically supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, and 30% glycerol .