Recombinant Proteins
Product List
CNOT7 Mouse
CCR4-NOT Transcription Complex, Subunit 7 Mouse Recombinant
CNOT7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
CNOT8 Human
CCR4-NOT Transcription Complex, Subunit 8 Human Recombinant
CNOT8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
The CCR4-NOT transcription complex is a multiprotein complex that plays a crucial role in gene expression regulation. It is involved in various cellular processes, including transcription, mRNA degradation, and protein modification. The complex has multiple enzymatic activities, such as poly(A) 3′-5′ exonuclease and ubiquitin ligase . It is conserved across eukaryotes and consists of several subunits, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, CNOT10, and CNOT11 .
The CCR4-NOT complex is highly conserved and multifunctional. It is present in both the nucleus and cytoplasm, where it regulates various aspects of mRNA metabolism . The complex is expressed in multiple tissues and has a broad tissue distribution. Key biological properties include its role in mRNA degradation, transcription initiation and elongation, ubiquitination, and protein modification .
The primary biological functions of the CCR4-NOT complex include:
- Regulation of mRNA stability and degradation: The complex is involved in deadenylation, which is the shortening of the poly(A) tail of mRNA, leading to mRNA decay .
- Transcription regulation: It plays a role in both the activation and inhibition of transcription initiation and elongation .
- Protein quality control: The complex is involved in ubiquitination, which tags proteins for degradation .
- Immune responses and pathogen recognition: The complex has been implicated in the regulation of immune responses and pathogen recognition, although specific mechanisms are still being studied .
The CCR4-NOT complex interacts with various molecules and cells through multiple mechanisms:
- Binding partners: The complex interacts with RNA-binding proteins, miRNA-bound mRNAs, and ribosomes .
- Downstream signaling cascades: It is involved in multiple signaling pathways that regulate mRNA stability, translation, and degradation .
- Transcription elongation: The complex promotes transcription elongation by interacting with elongating RNA polymerase II complexes .
The expression and activity of the CCR4-NOT complex are regulated through several mechanisms:
- Transcriptional regulation: The complex is regulated at the transcriptional level by various transcription factors .
- Post-translational modifications: The complex undergoes post-translational modifications, such as ubiquitination and phosphorylation, which modulate its activity .
- Recruitment to target mRNAs: The complex is recruited to target mRNAs through interactions with RNA-binding proteins and miRNA complexes .
The CCR4-NOT complex has several applications in biomedical research, diagnostics, and therapeutics:
- Biomedical research: It is studied for its role in gene expression regulation and mRNA metabolism .
- Diagnostic tools: The complex’s components can be used as biomarkers for various diseases .
- Therapeutic strategies: Targeting the complex’s activity can be a potential therapeutic strategy for diseases related to gene expression dysregulation .
The CCR4-NOT complex plays a role throughout the life cycle, from development to aging and disease:
