CNOT7 Mouse
Description
CNOT7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The CCR4-NOT transcription complex is a multi-subunit protein complex that plays a crucial role in the regulation of gene expression. It is involved in various cellular processes, including mRNA degradation, transcriptional regulation, and translational repression. One of the key components of this complex is the CCR4-NOT Transcription Complex, Subunit 7 (CNOT7), also known as CAF1 or BTG1-Binding Factor 1.
CNOT7 is a catalytic subunit of the CCR4-NOT complex and is known for its 3’-5’ poly(A) exoribonuclease activity . This activity is essential for the deadenylation of mRNA, a process that leads to mRNA degradation. The CCR4-NOT complex, including CNOT7, is involved in various cellular processes such as bulk mRNA degradation, miRNA-mediated repression, and general transcription regulation .
The CNOT7 gene undergoes alternative splicing, resulting in multiple transcript variants. These variants can lead to the production of different protein isoforms with distinct functions. For example, one of the isoforms, CNOT7v2, has been shown to interact with CCR4-NOT subunits but does not bind to BTG proteins . This isoform is involved in nuclear processes such as arginine methylation and alternative splicing, rather than mRNA turnover .
CNOT7 plays a significant role in regulating cell proliferation and the innate immune response. It binds to anti-proliferative proteins, such as B-cell translocation protein 1 (BTG1), which negatively regulates cell proliferation . This interaction is driven by the phosphorylation of the anti-proliferative protein, leading to changes in cell proliferation associated with cell-cell contact . Additionally, CNOT7 downregulates the innate immune response, providing a therapeutic target for enhancing antimicrobial activity against foreign agents .
The study of CNOT7 and its role in the CCR4-NOT complex has significant implications for understanding gene expression regulation and developing therapeutic strategies. For instance, the alternative splicing of CNOT7 and its interaction with other proteins can provide insights into the diversification of CCR4-NOT functions in different cell types and tissues . Moreover, the recombinant form of CNOT7 (Mouse) is used in various research applications to study its biochemical properties and interactions with other proteins.
