CNOT8 Human
Greater than 85.0% as determined by SDS-PAGE.
Description
CNOT8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The CCR4-NOT complex is composed of several subunits, each contributing to its diverse functions. The core components of the complex include:
- CNOT1: Acts as a scaffold, bringing together other subunits and effector proteins .
- CNOT2 and CNOT3: Structural components linked to the maintenance of embryonic stem cells .
- CNOT4: Functions as an E3 ubiquitin ligase .
- CNOT6 and CNOT6L: 3′-5′ exonucleases with a preference for polyadenylated mRNA substrates .
- CNOT7 and CNOT8: 3′-5′ exonucleases that are partially redundant .
- CNOT9, CNOT10, and CNOT11: Non-catalytic subunits that form specific modules .
CNOT8, also known as CCR4-NOT Transcription Complex, Subunit 8, is one of the 3′-5′ exonucleases within the complex. It plays a significant role in mRNA decay by shortening the poly(A) tail at the 3’ end of eukaryotic mRNAs . This activity is crucial for the regulation of mRNA stability and, consequently, gene expression.
The CCR4-NOT complex is involved in multiple regulatory processes:
- mRNA Degradation: The complex shortens the poly(A) tail of mRNAs, leading to their degradation .
- Transcription Regulation: It regulates transcription initiation and elongation, impacting gene expression at the transcriptional level .
- Ubiquitination: The E3 ubiquitin ligase activity of CNOT4 within the complex tags proteins for degradation .
- Protein Modification: The complex is involved in various protein modification processes, influencing protein function and stability .
The CCR4-NOT complex is essential for maintaining cellular homeostasis and responding to environmental changes. Its role in mRNA metabolism ensures that gene expression is tightly regulated, allowing cells to adapt to dynamic conditions. The complex’s involvement in protein quality control and mRNA decay highlights its importance in cellular function and health .
In summary, the CCR4-NOT transcription complex, particularly Subunit 8 (CNOT8), is a vital component of the cellular machinery that regulates gene expression through various mechanisms. Its multifunctional nature and evolutionary conservation underscore its significance in eukaryotic biology.
