Recombinant Proteins
Product List
ANG Human
Angiogenin Human Recombinant
ANG is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
ANG Human, Sf9
ANG Human Recombinant, Sf9
ANG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 132 amino acids (25-147a.a.) and having a molecular mass of 15.2kDa.
ANG is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Key Biological Properties: Angiogenin exhibits weak ribonucleolytic activity, which is crucial for its biological functions . It hydrolyzes cellular RNA, modulating protein synthesis levels and interacting with DNA to increase rRNA expression .
Expression Patterns and Tissue Distribution: Angiogenin is widely expressed in various human tissues and fluids, including plasma, amniotic fluid, tumor microenvironment, and cerebrospinal fluid . It is present in normal human tissues and fluids, such as plasma and amniotic fluid .
Primary Biological Functions: Angiogenin plays a key role in angiogenesis, the formation of new blood vessels, which is essential for normal and tumor growth . It interacts with endothelial and smooth muscle cells, resulting in cell migration, invasion, proliferation, and formation of tubular structures .
Role in Immune Responses and Pathogen Recognition: Angiogenin exhibits wound healing properties and microbicidal activity, contributing to host immunity . It is involved in regulating cell proliferation, survival, migration, invasion, and differentiation .
Mechanisms with Other Molecules and Cells: Angiogenin binds to actin of both smooth muscle and endothelial cells to form complexes that activate proteolytic cascades, upregulating the production of proteases and plasmin . These enzymes degrade the laminin and fibronectin layers of the basement membrane, allowing endothelial cells to penetrate and migrate into the perivascular tissue .
Binding Partners and Downstream Signaling Cascades: Angiogenin activates signal transduction pathways at the cellular membrane of endothelial cells, producing extracellular signal-related kinase1/2 (ERK1/2) and protein kinase B/Akt . These proteins lead to the invasion of the basement membrane and cell proliferation associated with further angiogenesis .
Transcriptional Regulation and Post-Translational Modifications: Angiogenin’s expression and activity are regulated by various mechanisms. It binds to actin to form complexes that activate proteolytic cascades, upregulating the production of proteases and plasmin . Under stress conditions, angiogenin translocates to the cytosol and cleaves tRNA into fragments, inhibiting ribosome biogenesis and protein synthesis .
Biomedical Research: Angiogenin has been extensively studied in cancer and neurodegenerative diseases due to its role in angiogenesis and gene expression regulation .
Diagnostic Tools and Therapeutic Strategies: Angiogenin’s unique properties make it a potential target for diagnostic tools and therapeutic strategies. It has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, and epidermal growth factor .
Development to Aging and Disease: Angiogenin plays a crucial role throughout the life cycle, from development to aging and disease. It is involved in various physiological and pathological processes, including cell proliferation, survival, migration, invasion, and differentiation . Angiogenin’s role in angiogenesis is essential for normal development and tumor growth .
