ANG Human, Sf9
Sf9, Baculovirus cells.
Angiogenin, ANG, Ribonuclease 5, RNase 5, RNASE5, ribonuclease, RNase A family, 5, ALS9, HEL168, MGC22466, MGC71966, RNASE4, RAA1.
Greater than 90% as determined by SDS-PAGE.
Description
ANG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 132 amino acids (25-147a.a.) and having a molecular mass of 15.2kDa.
ANG is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Angiogenin (ANG), a member of the RNase A family, plays a crucial role in the formation of new blood vessels (angiogenesis). It achieves this by cleaving tRNA at specific sites (anticodon loops), generating tRNA-derived stress-induced fragments (tiRNAs). These tiRNAs have been shown to suppress protein synthesis and initiate the formation of stress granules. Additionally, ANG contributes to the synthesis of ribosomal RNA. Its interaction with RNH1, observed in living organisms (in vivo), is vital for regulating its angiogenic activity.
Produced using Sf9 insect cells (specifically, Baculovirus expression system), our ANG protein is a single, glycosylated polypeptide chain comprising 132 amino acids (spanning positions 25 to 147a.a.). It has a molecular weight of 15.2 kDa. For ease of purification and detection, a 6-amino acid Histidine tag (His-Tag) is fused to the C-terminus of the protein. Purification is carried out using proprietary chromatographic techniques.
The ANG protein is supplied in a solution containing 0.25mg/ml of ANG protein, 10% glycerol, and Phosphate Buffered Saline (pH 7.4).
The purity of our ANG protein is greater than 90% as determined by SDS-PAGE analysis.
Angiogenin, ANG, Ribonuclease 5, RNase 5, RNASE5, ribonuclease, RNase A family, 5, ALS9, HEL168, MGC22466, MGC71966, RNASE4, RAA1.
Sf9, Baculovirus cells.
ADPQDNSRYT HFLTQHYDAK PQGRDDRYCE SIMRRRGLTS PCKDINTFIH GNKRSIKAIC ENKNGNPHRE NLRISKSSFQ VTTCKLHGGS PWPPCQYRAT AGFRNVVVAC ENGLPVHLDQ SIFRRPHHHH HH.
Product Science Overview
ANG produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 132 amino acids (25-147a.a.) and has a molecular mass of approximately 15.2 kDa . The molecular size on SDS-PAGE appears at approximately 13.5-18 kDa . This recombinant form of ANG is fused to a 9 amino acid His-Tag at the C-terminus, which facilitates its purification through chromatographic techniques .
ANG cleaves transfer RNA (tRNA) within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs). These tiRNAs inhibit protein synthesis and trigger the assembly of stress granules, which are involved in cellular stress responses . Additionally, ANG stimulates ribosomal RNA synthesis, contributing to its role in promoting cell growth and survival .
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Angiogenesis: ANG is a highly effective mediator of new blood vessel formation. This function is critical in processes such as wound healing and tumor growth, where the formation of new blood vessels is essential for supplying nutrients and oxygen to tissues .
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Neuroprotection: ANG has been shown to have neuroprotective effects, particularly in the context of neurodegenerative diseases. It helps in the survival of motor neurons and has been implicated in conditions like amyotrophic lateral sclerosis (ALS) .
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Stress Response: By producing tiRNAs, ANG plays a role in the cellular response to stress. These fragments help in the formation of stress granules, which are involved in protecting cells under adverse conditions .
The recombinant form of ANG produced in Sf9 cells is primarily used for laboratory research purposes. It is utilized in studies related to angiogenesis, cancer research, and neuroprotection. The protein is supplied as a sterile, filtered, colorless solution and is formulated with 10% glycerol and phosphate-buffered saline (pH 7.4) .
