ANG Human
Greater than 85.0% as determined by SDS-PAGE.
Description
ANG is fused to a 21 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Angiogenin is a 14-kilodalton ribonuclease enzyme that plays a crucial role in various physiological and pathological processes. It is involved in tumorigenesis, neuroprotection, inflammation, innate immunity, reproduction, tissue regeneration, and cellular stress responses . Human recombinant angiogenin is a synthetically produced version of this enzyme, designed to mimic its natural counterpart.
Angiogenin was first discovered in 1985 by Bert L. Vallee and his colleagues. It is a member of the ribonuclease A superfamily and shares structural similarities with other ribonucleases. The enzyme consists of 123 amino acids and has a molecular weight of approximately 14 kDa . Its three-dimensional structure includes a characteristic ribonuclease fold, which is essential for its enzymatic activity.
Angiogenin is known for its ability to induce angiogenesis, the process of forming new blood vessels from pre-existing ones. This function is critical for wound healing, tissue regeneration, and the growth of tumors. Angiogenin achieves this by binding to specific receptors on endothelial cells, stimulating their proliferation and migration .
In addition to its role in angiogenesis, angiogenin has several other biological functions:
- Neuroprotection: Angiogenin has been shown to protect neurons from apoptosis (programmed cell death) and promote neuronal survival under stress conditions .
- Inflammation and Immunity: Angiogenin modulates the immune response and has anti-inflammatory properties .
- Stress Response: Under stress conditions, angiogenin accumulates in the cytoplasm and modulates the production of transfer RNA-derived stress-induced RNAs (tiRNAs), which contribute to translational inhibition and the formation of stress granules .
Human recombinant angiogenin is produced using recombinant DNA technology, typically in Escherichia coli (E. coli) expression systems. The recombinant protein is purified to high levels of purity, often exceeding 97% . It retains the biological activities of the natural enzyme, including its ribonucleolytic activity and ability to induce angiogenesis .
Recombinant angiogenin has several potential clinical and research applications:
- Cancer Therapy: Due to its role in angiogenesis, angiogenin is a target for anti-cancer therapies aimed at inhibiting tumor growth by blocking blood vessel formation .
- Neurodegenerative Diseases: Angiogenin’s neuroprotective properties make it a potential therapeutic agent for neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and Alzheimer’s disease .
- Wound Healing: Recombinant angiogenin can be used to promote wound healing and tissue regeneration in various medical conditions .
