Recombinant Proteins
Product List
Ag85A
Mycobacterium Tuberculosis major secretory protein Antigen 85A Recombinant
Recombinant Mycobacterium tuberculosis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total).
Antigen 85A is fused to a 6 amino acid His tag at C-terminus and purified by conventional chromatographic techniques.
Ag85B
Mycobacterium Tuberculosis major secretory protein Antigen 85B Recombinant
Introduction
Ag85, also known as the antigen 85 complex, is a group of proteins secreted by Mycobacterium tuberculosis. This complex consists of three main proteins: Ag85A, Ag85B, and Ag85C. These proteins are mycolyltransferases, which play a crucial role in the synthesis of the mycobacterial cell wall by transferring mycolic acids to the cell wall components .
Key Biological Properties: Ag85 proteins are highly conserved and are among the most abundantly secreted proteins by M. tuberculosis. They exhibit mycolyltransferase activity, which is essential for cell wall biosynthesis .
Expression Patterns: Ag85 proteins are expressed during the early phase of M. tuberculosis infection and are continuously secreted throughout the infection process .
Tissue Distribution: These proteins are primarily found in the cell wall of M. tuberculosis and are secreted into the surrounding environment, where they interact with host cells .
Primary Biological Functions: The primary function of Ag85 proteins is to catalyze the transfer of mycolic acids to the cell wall components, which is essential for maintaining the integrity and functionality of the mycobacterial cell wall .
Role in Immune Responses: Ag85 proteins are recognized by the host immune system and play a significant role in eliciting an immune response. They are potent antigens that stimulate both humoral and cellular immune responses .
Pathogen Recognition: Ag85 proteins are involved in the recognition and binding of host fibronectin, which facilitates the adherence of M. tuberculosis to host cells and aids in the establishment of infection .
Mechanisms with Other Molecules and Cells: Ag85 proteins interact with various host molecules, including fibronectin, to mediate the adherence of M. tuberculosis to host cells . They also interact with other mycobacterial proteins to facilitate cell wall biosynthesis .
Binding Partners: The primary binding partners of Ag85 proteins are mycolic acids and fibronectin .
Downstream Signaling Cascades: Upon binding to host cells, Ag85 proteins can trigger downstream signaling cascades that modulate the host immune response and promote the survival of M. tuberculosis within host cells .
Regulatory Mechanisms: The expression and activity of Ag85 proteins are tightly regulated at both the transcriptional and post-translational levels .
Transcriptional Regulation: The transcription of Ag85 genes is regulated by various transcription factors that respond to environmental cues and stress conditions .
Post-Translational Modifications: Ag85 proteins undergo post-translational modifications, such as glycosylation, which can affect their stability, activity, and interactions with other molecules .
Biomedical Research: Ag85 proteins are extensively studied in the context of tuberculosis research. They serve as important targets for the development of new diagnostic tools and therapeutic strategies .
Diagnostic Tools: Ag85 proteins are used in various diagnostic assays, such as ELISA and PCR, to detect M. tuberculosis infection .
Therapeutic Strategies: Ag85 proteins are being explored as potential targets for the development of new vaccines and antimicrobial agents against tuberculosis .
Role Throughout the Life Cycle: Ag85 proteins play a critical role throughout the life cycle of M. tuberculosis. They are essential for the initial establishment of infection, the maintenance of the cell wall during replication, and the persistence of the bacterium within host cells .
From Development to Aging and Disease: The continuous expression and secretion of Ag85 proteins are crucial for the survival and pathogenicity of M. tuberculosis at all stages of its life cycle .
