Recombinant Proteins
Product List
OmpA
Outer Membrane Protein-A Bacterial Recombinant
The recombinant form was found to be undistinguishable from the wild type when examined by SDS-PAGE and gel filtration chromatography yielding a 50.5 kDa monomeric protein. The immunological similarity of the protein samples was demonstrated by employing polyclonal and monoclonal antibodies in ELISA and Western Blot techniques. All forms of A-protein were found to activate the secretion of tumour necrosis factor alpha from murine macrophage. For ref see Maurice et al. (1999) Protein Expression and Purification 16, 396-404.
The OmpA is purified by proprietary chromatographic techniques.
Introduction
Outer Membrane Proteins (OMPs) are integral membrane proteins located in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. They play crucial roles in various cellular processes. OMPs can be classified into several categories based on their structure and function:
- Porins: Form channels that allow the passive diffusion of small molecules.
- Transporters: Facilitate the active transport of specific substrates.
- Enzymes: Catalyze biochemical reactions.
- Receptors: Involved in signal transduction and communication with the environment.
Key Biological Properties:
- Structure: Typically β-barrel structures that span the membrane.
- Stability: Highly stable due to their β-barrel configuration.
- Hydrophobicity: Hydrophobic exterior interacts with the lipid bilayer, while the interior is often hydrophilic.
Expression Patterns:
- OMPs are expressed in a regulated manner, often in response to environmental conditions.
Tissue Distribution:
- Found in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts.
Primary Biological Functions:
- Nutrient Uptake: Facilitate the uptake of essential nutrients.
- Waste Removal: Help in the expulsion of waste products.
- Structural Integrity: Contribute to the structural integrity of the outer membrane.
Role in Immune Responses:
- Pathogen Recognition: Recognized by the host immune system, triggering immune responses.
- Antigen Presentation: Some OMPs act as antigens, eliciting an immune response.
Mechanisms with Other Molecules and Cells:
- Binding Partners: Interact with various molecules such as lipopolysaccharides, peptidoglycans, and other proteins.
- Downstream Signaling Cascades: Trigger signaling pathways that lead to cellular responses.
Control of Expression and Activity:
- Transcriptional Regulation: Controlled by transcription factors and environmental signals.
- Post-Translational Modifications: Modifications such as phosphorylation and glycosylation can affect their function and stability.
Biomedical Research:
- Model Systems: Used as models to study membrane protein structure and function.
- Drug Targets: Potential targets for antibiotics and other therapeutic agents.
Diagnostic Tools:
- Biomarkers: Serve as biomarkers for the detection of bacterial infections.
Therapeutic Strategies:
- Vaccine Development: Used in the development of vaccines against bacterial pathogens.
Throughout the Life Cycle:
- Development: Essential for the proper development and functioning of bacterial cells.
- Aging: Changes in OMP expression and function can occur with aging.
- Disease: Alterations in OMPs are associated with various diseases, including bacterial infections and mitochondrial disorders.
