Recombinant Proteins
Product List
B.Microti IRA
Babesia Microti IRA Recombinant
B.Microti IRA is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
B.Microti p32
Babesia Microti p32 Recombinant
B.Microti p32 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
B.Microti p41
Babesia Microti p41 Recombinant
Recombinant Babesia Microti p41 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 38kDa.
B.Microti p41 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction
Babesia microti is a parasitic blood-borne piroplasm transmitted by deer ticks (Ixodes scapularis). It is responsible for the disease babesiosis, a malaria-like illness characterized by fever and hemolysis . Babesia microti belongs to the phylum Apicomplexa, class Aconoidasida, and order Piroplasmida .
Key Biological Properties: Babesia microti is an intraerythrocytic parasite, meaning it invades and resides within red blood cells (RBCs). It is primarily transmitted through tick bites but can also be spread via blood transfusion and organ transplantation .
Expression Patterns and Tissue Distribution: The parasite is found in the blood, specifically within RBCs. It undergoes asexual reproduction within these cells, leading to their eventual rupture and the release of new parasites .
Primary Biological Functions: Babesia microti’s primary function is to invade and replicate within RBCs. This invasion and replication process is crucial for its survival and propagation .
Role in Immune Responses and Pathogen Recognition: The parasite elicits an immune response from the host, including the production of specific antibodies. It can evade the immune system through various mechanisms, including altering its surface proteins .
Mechanisms with Other Molecules and Cells: Babesia microti interacts with host cells through surface proteins that facilitate its entry into RBCs. These interactions are critical for the parasite’s invasion and survival .
Binding Partners and Downstream Signaling Cascades: The parasite’s surface proteins bind to receptors on the host cell membrane, triggering signaling cascades that allow the parasite to enter and replicate within the cell .
Regulatory Mechanisms Controlling Expression and Activity: The expression of Babesia microti’s genes is tightly regulated to ensure its survival and replication within the host. This regulation involves both transcriptional and post-translational modifications .
Transcriptional Regulation and Post-Translational Modifications: Specific transcription factors and signaling pathways control the expression of genes involved in the parasite’s life cycle. Post-translational modifications, such as phosphorylation, play a role in regulating the activity of key proteins .
Diagnostic Tools: Babesia microti antigens are used in diagnostic assays to detect the presence of the parasite in blood samples. These assays include PCR and serological tests .
Therapeutic Strategies: Research is ongoing to develop vaccines and therapeutic agents targeting Babesia microti. Some promising candidates include surface antigens that elicit protective immune responses .
Role Throughout the Life Cycle: Babesia microti’s life cycle involves two hosts: a rodent (primarily the white-footed mouse) and a tick (Ixodes scapularis). The parasite undergoes asexual reproduction within the rodent’s RBCs and sexual reproduction within the tick . Humans can become accidental hosts through tick bites, leading to babesiosis .
