B.Microti p32
Babesia Microti p32 Recombinant
Cat. No.
BT25412
Source
Sf9 insect cells.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Recombinant Babesia Microti p32 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 35,808 Dalton.
B.Microti p32 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
B.Microti p32 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Babesiosis, a disease caused by apicomplexan parasites of the Babesia genus, primarily affects rodents like the white-footed mouse. The Babesia microti life cycle involves both a rodent and a tick from the Ixodes genus. An infected tick transmits sporozoites to the mouse during feeding, which reproduce asexually within red blood cells. Some parasites differentiate into gametes, but these are indistinguishable under a light microscope. Ticks become definitive hosts upon ingesting infected blood, where gametes combine and undergo sporogony, producing sporozoites. While transovarial transmission is observed in larger Babesia species, it's absent in smaller ones like B. microti. Humans become accidental hosts through tick bites, with sporozoites entering red blood cells and replicating asexually. This multiplication causes the disease's symptoms. Humans are typically dead-end hosts, but transmission through blood transfusions is possible.
Description
Recombinant Babesia Microti p32, expressed in SF9 cells, is a glycosylated polypeptide with a molecular weight of 35,808 Daltons. This protein features a 10xHis tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
B.Microti p32 is supplied in a solution containing 20mM HEPES buffer (pH 7.6), 250mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 80% using SDS-PAGE analysis.
Source
Sf9 insect cells.
Product Science Overview
Introduction
Babesia microti is a protozoan parasite that causes babesiosis, a zoonotic disease transmitted primarily through tick bites. This parasite infects the erythrocytes (red blood cells) of its mammalian hosts, leading to a range of symptoms from mild flu-like illness to severe, life-threatening conditions, particularly in immunocompromised individuals .
Babesia Microti p32 Protein
Recombinant p32 Protein
Recombinant proteins are proteins that are genetically engineered in the laboratory. The recombinant p32 protein of Babesia microti is produced by inserting the gene encoding the p32 protein into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production and purification of the protein for research and diagnostic applications .
Applications in Research and Diagnostics
- Diagnostic Tool: The recombinant p32 protein can be used in serological assays to detect antibodies against Babesia microti in infected individuals. This is particularly useful for diagnosing asymptomatic or mild cases of babesiosis, where traditional microscopic examination may not be sensitive enough .
- Vaccine Development: Research has shown that immunization with recombinant proteins can elicit an immune response that provides partial protection against infection. The p32 protein is being investigated as a potential vaccine candidate to prevent babesiosis .
- Understanding Pathogenesis: Studying the recombinant p32 protein helps researchers understand the mechanisms by which Babesia microti invades and survives within host erythrocytes. This knowledge is crucial for developing targeted therapies to combat the infection .
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