Recombinant Proteins
Product List
MED20 Human
Mediator Complex Subunit 20 Human Recombinant
MED20 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 235 amino acids (1-212 a.a.) and having a molecular mass of 25.6kDa.
MED20 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MED21 Human
Mediator Complex Subunit 21 Human Recombinant
MED21 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 18.0kDa. MED21 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MED27 Human
Mediator Complex Subunit 27 Human Recombinant
MED27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MED4 Human
Mediator Complex Subunit 4 Human Recombinant
MED7 Human
Mediator Complex Subunit 7 Human Recombinant
MED7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
The Mediator complex is a multiprotein complex that functions as a transcriptional coactivator in all eukaryotes. It was discovered in 1990 in the lab of Roger D. Kornberg, who received the 2006 Nobel Prize in Chemistry for his work on the molecular basis of eukaryotic transcription . The Mediator complex interacts with transcription factors and RNA polymerase II, transmitting signals from the transcription factors to the polymerase . It is highly variable in its evolutionary, compositional, and conformational levels .
Key Biological Properties: The Mediator complex is essential for the successful transcription of nearly all class II gene promoters in yeast and works similarly in mammals . It is composed of 25 subunits in yeast and up to 26 subunits in mammals .
Expression Patterns and Tissue Distribution: The Mediator complex is located within the cell nucleus and is ubiquitously expressed in various tissues . Its subunits can be differentially expressed in specific tissues or developmental stages, contributing to its diverse functions .
Primary Biological Functions: The Mediator complex serves as a bridge between RNA polymerase II and transcription factors, facilitating the assembly of the pre-initiation complex (PIC) and enabling transcription initiation . It plays a crucial role in regulating gene expression in response to various signals .
Role in Immune Responses and Pathogen Recognition: The Mediator complex is involved in the transcriptional regulation of genes associated with immune responses and pathogen recognition. It helps in the activation of immune-related genes in response to environmental stresses .
Mechanisms with Other Molecules and Cells: The Mediator complex interacts with various transcription factors, coactivators, and general transcription factors to regulate gene expression . It undergoes conformational changes to facilitate the assembly of the PIC and the recruitment of RNA polymerase II .
Binding Partners and Downstream Signaling Cascades: The Mediator complex binds to the C-terminal domain of RNA polymerase II holoenzyme and acts as a bridge between this enzyme and transcription factors . It also interacts with enhancer-bound coactivators to establish enhancer-promoter contact, facilitating transcriptional activation .
Transcriptional Regulation: The Mediator complex modulates the association between transcription factors and RNA polymerase II to precisely regulate gene transcription . It is involved in the regulation of enhancer-promoter interactions and the formation of transcriptional hubs .
Post-Translational Modifications: The activity of the Mediator complex is regulated by various post-translational modifications, including phosphorylation by cyclin-dependent kinases (CDKs) . These modifications can alter the conformation and function of the complex, affecting its ability to regulate gene expression .
Biomedical Research: The Mediator complex is a key target in biomedical research due to its central role in transcriptional regulation. It is studied for its involvement in various diseases, including cancer and developmental disorders .
Diagnostic Tools and Therapeutic Strategies: Understanding the function and regulation of the Mediator complex can lead to the development of diagnostic tools and therapeutic strategies. Targeting specific subunits or regulatory mechanisms of the complex may provide new avenues for treating diseases associated with dysregulated gene expression .
Development to Aging and Disease: The Mediator complex plays a crucial role throughout the life cycle, from development to aging and disease. It is involved in the regulation of developmental transitions, such as vegetative phase change and floral transition in plants . In mammals, it regulates gene expression during various stages of development and is implicated in age-related diseases and cancer .
