MED7 Human

Mediator Complex Subunit 7 Human Recombinant
Cat. No.
BT6065
Source
E.coli.
Synonyms
Mediator complex subunit 7, cofactor required for Sp1 transcriptional activation subunit 9 33kDa, Activator-recruited cofactor 34 kDa component, Transcriptional coactivator CRSP33, RNA polymerase transcriptional regulation mediator subunit 7 homolog, mediator of RNA polymerase II transcription subunit 7, CRSP complex subunit 9, CRSP33, CRSP9, ARC34, hMED7.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
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Description

MED7 Human Recombinant produced in E. coli is a single polypeptide chain containing 256 amino acids (1-233) and having a molecular mass of 29.7 kDa.
MED7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MED7, a member of the Mediator complex subunit 7 family, plays a crucial role in gene transcription activation. This process involves transcription factors recognizing enhancer sites on DNA and collaborating with co-activators to initiate transcription by RNA polymerase II. MED7 is a component of the CRSP complex, which, along with TFIID, is essential for efficient activation by SP1. Moreover, it participates in other multisubunit complexes like thyroid hormone receptor-associated proteins, enabling TR function on DNA templates in conjunction with initiation factors and cofactors.
Description
Recombinant human MED7, produced in E. coli, is a single polypeptide chain consisting of 256 amino acids (1-233) with a molecular weight of 29.7 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The MED7 solution is provided at a concentration of 0.5 mg/mL in 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 85% as determined by SDS-PAGE analysis.
Synonyms
Mediator complex subunit 7, cofactor required for Sp1 transcriptional activation subunit 9 33kDa, Activator-recruited cofactor 34 kDa component, Transcriptional coactivator CRSP33, RNA polymerase transcriptional regulation mediator subunit 7 homolog, mediator of RNA polymerase II transcription subunit 7, CRSP complex subunit 9, CRSP33, CRSP9, ARC34, hMED7.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGEPQQV SALPPPPMQY IKEYTDENIQ EGLAPKPPPP IKDSYMMFGN QFQCDDLIIR PLESQGIERL HPMQFDHKKE LRKLNMSILI NFLDLLDILI RSPGSIKREE KLEDLKLLFV HVHHLINEYR PHQARETLRV MMEVQKRQRL ETAERFQKHL ERVIEMIQNC LASLPDDLPH SEAGMRVKTE PMDADDSNNC TGQNEHQREN SGHRRDQIIE KDAALCVLID EMNERP

Product Science Overview

Introduction

The Mediator complex is a crucial component of the RNA polymerase II (Pol II) transcription machinery in eukaryotic cells. It serves as a bridge, facilitating communication between gene-specific transcription factors and the Pol II enzyme, thereby regulating gene expression. One of the key subunits of this complex is Mediator Complex Subunit 7 (MED7).

Structure and Function

MED7 is a protein-coding gene that plays a significant role in transcriptional regulation. It is part of the central module of the Mediator complex, which is essential for the assembly and stability of the entire complex . MED7 interacts with other subunits within the Mediator complex to form a scaffold that supports the binding of transcription factors and Pol II .

Biological Significance

MED7 is involved in various biological processes, including:

  • Transcriptional Regulation: MED7 acts as a coactivator, enhancing the transcription of specific genes by facilitating the interaction between transcription factors and Pol II .
  • Cell Differentiation: MED7 is implicated in the regulation of cell differentiation, particularly in white adipocyte differentiation .
  • Stress Response: Studies have shown that MED7 is responsive to various stress conditions, such as heavy metal exposure, low temperature, and hormonal treatments .
Clinical Relevance

MED7 has been identified as a potential biomarker for certain types of cancer. For instance, high MED7 expression is associated with a good prognosis in invasive breast cancer, particularly in estrogen receptor-positive (ER+) luminal subtypes . This suggests that MED7 could be used as a prognostic marker to predict patient outcomes and tailor treatment strategies.

Recombinant MED7

Human recombinant MED7 is produced using recombinant DNA technology, which involves inserting the MED7 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used in various research applications, including:

  • Structural Studies: Understanding the structure of MED7 and its interactions with other Mediator subunits.
  • Functional Assays: Investigating the role of MED7 in transcriptional regulation and other cellular processes.
  • Drug Development: Screening for potential therapeutic compounds that target MED7 or its interactions within the Mediator complex.

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