MED27 Human

Mediator Complex Subunit 27 Human Recombinant
Cat. No.
BT5955
Source
E.coli.
Synonyms
Mediator Complex Subunit 27, MED27, CRSP8, Cofactor Required For Sp1 Transcriptional Activation, Subunit 8 34kDa, P37 TRAP/SMCC/PC2 Subunit, Transcriptional Coactivator CRSP34, CRSP34, CRSP Complex Subunit 8, CRAP34, TRAP37, Mediator of RNA Polymerase II Transcription Subunit 27, Cofactor Required For Sp1 Transcriptional Activation Subunit 8.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MED27 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 334 amino acids (1-311) and having a molecular mass of 37.8 kDa.
MED27 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mediator Complex Subunit 27 (MED27) is a crucial component of the CRSP complex, working alongside TFIID to facilitate efficient gene activation by SP1. As part of the Mediator complex, a key coactivator, MED27 plays a vital role in regulating the transcription of nearly all genes dependent on RNA polymerase II. This protein is also found in other multi-protein complexes, including those associated with the thyroid hormone receptor (TR). These complexes, along with initiation factors and cofactors, interact with TR to support its function on DNA templates. The activation of the MED27 gene is a complex, multi-step process initiated by factors that recognize specific enhancer sites within DNA.
Description
Recombinant human MED27, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 334 amino acids, with a sequence spanning residues 1-311, and has a molecular weight of 37.8 kDa. A 23 amino acid His-tag is fused to the N-terminus of MED27 to aid in purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MED27 solution is prepared at a concentration of 0.5 mg/ml and contains the following components: 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the MED27 solution should be kept at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of MED27 is determined by SDS-PAGE analysis and is consistently greater than 80%.
Synonyms
Mediator Complex Subunit 27, MED27, CRSP8, Cofactor Required For Sp1 Transcriptional Activation, Subunit 8 34kDa, P37 TRAP/SMCC/PC2 Subunit, Transcriptional Coactivator CRSP34, CRSP34, CRSP Complex Subunit 8, CRAP34, TRAP37, Mediator of RNA Polymerase II Transcription Subunit 27, Cofactor Required For Sp1 Transcriptional Activation Subunit 8.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADVINV SVNLEAFSQA ISAIQALRSS VSRVFDCLKD GMRNKETLEG REKAFIAHFQ DNLHSVNRDL NELERLSNLV GKPSENHPLH NSGLLSLDPV QDKTPLYSQL LQAYKWSNKL QYHAGLASGL LNQQSLKRSA NQMGVSAKRR PKAQPTTLVL PPQYVDDVIS RIDRMFPEMS IHLSRPNGTS AMLLVTLGKV LKVIVVMRSL FIDRTIVKGY NENVYTEDGK LDIWSKSNYQ VFQKVTDHAT TALLHYQLPQ MPDVVVRSFM TWLRSYIKLF QAPCQRCGKF LQDGLPPTWR DFRTLEAFHD TCRQ.

Product Science Overview

Introduction

Mediator Complex Subunit 27 (MED27), also known as CRAP34, CRSP34, CRSP8, TRAP37, and MED3, is a protein encoded by the MED27 gene in humans . This protein is a crucial component of the Mediator complex, which plays a significant role in the regulation of transcription by RNA polymerase II .

Structure and Function

The Mediator complex is a multiprotein complex that functions as a transcriptional coactivator in all eukaryotes. MED27 is one of the subunits of this complex and is involved in the regulation of gene transcription. The Mediator complex acts as a bridge, conveying information from gene-specific regulatory proteins to the RNA polymerase II transcription machinery .

MED27 is ubiquitously expressed, indicating its essential role in transcriptional initiation across various cell types . The protein encoded by the MED27 gene is involved in the activation of gene transcription, a multistep process triggered by factors that recognize transcriptional enhancer sites in DNA .

Biological Processes

MED27 is involved in several biological processes, including:

  • Regulation of Transcription by RNA Polymerase II: MED27 is a part of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1 .
  • Stem Cell Population Maintenance: MED27 plays a role in maintaining stem cell populations .
  • Protein Ubiquitination: MED27 is involved in the process of protein ubiquitination, which is crucial for protein degradation and regulation .
Clinical Significance

The MED27 gene has been studied for its role in various diseases and conditions. For instance, loss of CRSP34/MED27 decreases the number of amacrine cells but increases the number of rod photoreceptor cells . This suggests that MED27 may have a role in the development and function of the retina.

Recombinant MED27

Recombinant MED27 refers to the MED27 protein that is produced through recombinant DNA technology. This involves inserting the MED27 gene into an expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant MED27 is used in various research applications to study its function and role in transcriptional regulation.

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