MED4 Human
Description
Product Specs
MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD LEHHHHHH.
Product Science Overview
The Mediator complex is a large, multi-protein complex that is evolutionarily conserved across eukaryotes. It plays a crucial role in the regulation of transcription by RNA polymerase II. The complex is composed of multiple subunits, each contributing to its overall function. One of these subunits is Mediator Complex Subunit 4 (MED4), which is also known as Human Recombinant MED4 when produced through recombinant DNA technology.
The Mediator complex was initially identified in yeast in the early 1990s by the Kornberg and Young laboratories . The complex was found to stimulate activator-dependent transcription in vitro and was tightly bound to RNA polymerase II. The Mediator complex in humans consists of approximately 30 subunits, which are organized into three main modules: Head, Middle, and Tail, along with a separable four-subunit kinase module .
MED4 is a component of the Middle module of the Mediator complex. It plays a significant role in bridging the interactions between the other subunits and RNA polymerase II. MED4 is essential for the proper assembly and stability of the Mediator complex, thereby facilitating the transcriptional activation of various genes.
Human Recombinant MED4 is produced using recombinant DNA technology, which involves inserting the gene encoding MED4 into an expression vector. This vector is then introduced into a host cell, such as E. coli or yeast, where the protein is expressed and subsequently purified. The recombinant production of MED4 allows for the study of its structure and function in a controlled environment, providing valuable insights into its role within the Mediator complex.
Research on MED4 and the Mediator complex has advanced significantly over the past two decades. Structural studies using techniques like cryo-electron microscopy (cryo-EM) have revealed detailed insights into the organization and interactions of Mediator subunits . These studies have highlighted the importance of MED4 in maintaining the integrity of the complex and its role in transcriptional regulation.
The recombinant production of MED4 has also facilitated various applications in biomedical research. For instance, it allows for the investigation of MED4’s role in disease mechanisms, the development of potential therapeutic targets, and the exploration of its interactions with other proteins and nucleic acids.
