MED21 Human
Description
MED21 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-144 a.a.) and having a molecular mass of 18.0kDa. MED21 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
This product consists of the human MED21 protein, which has been recombinantly produced in E. coli bacteria. The protein is not glycosylated, meaning it does not have sugar molecules attached to it. It comprises a single polypeptide chain of 167 amino acids, with the first 144 amino acids corresponding to the MED21 sequence. A 23 amino acid His-tag is attached to the N-terminus of the protein for purification purposes. The molecular weight of the protein is 18.0 kDa. The protein has been purified using proprietary chromatographic techniques.
Adding a carrier protein like HSA or BSA to a final concentration of 0.1% is advisable for long-term storage.
Repeated freezing and thawing of the solution should be avoided.
Product Science Overview
The Mediator complex is a large, multi-protein complex that plays a crucial role in the regulation of transcription in eukaryotic cells. It serves as a bridge between gene-specific transcription factors and the RNA polymerase II enzyme, facilitating the transcription of genetic information from DNA to RNA. The Mediator complex is composed of multiple subunits, each with specific functions. One of these subunits is Mediator Complex Subunit 21 (MED21), also known as SRB7 in yeast.
The Mediator complex in humans consists of 26 subunits, organized into four distinct modules: the head, middle, tail, and kinase modules . MED21 is a part of the head module, which is essential for the interaction with RNA polymerase II and the initiation of transcription . The head module is responsible for the recruitment of RNA polymerase II to the promoter regions of genes, thereby playing a critical role in the regulation of gene expression.
MED21, like other subunits of the Mediator complex, is involved in the regulation of transcription by serving as a coactivator. It interacts with various transcription factors and helps in the assembly of the pre-initiation complex at the promoter regions of genes . This interaction is crucial for the proper initiation and regulation of transcription, ensuring that genes are expressed at the right time and in the right amounts.
The Mediator complex, including MED21, is essential for the regulation of transcription by RNA polymerase II. It facilitates the communication between transcription factors bound to enhancer regions and the RNA polymerase II enzyme at the promoter regions . This communication is vital for the proper initiation, elongation, and termination of transcription. MED21, as part of the head module, plays a key role in this process by helping to stabilize the interaction between the Mediator complex and RNA polymerase II .
Recombinant DNA technology has enabled the production of human recombinant MED21, which is used in various research applications. Human recombinant MED21 is produced by inserting the gene encoding MED21 into a suitable expression system, such as bacteria or yeast, allowing for the production of large quantities of the protein. This recombinant protein can be used in biochemical and structural studies to better understand the function and regulation of the Mediator complex .
