Recombinant Proteins
Product List
GPNMB Human
Glycoprotein Nmb Human Recombinant
GPNMB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
GPNMB Human, HEK
Glycoprotein Nmb Human Recombinant, HEK
The Human GPNMB is purified by proprietary chromatographic techniques.
GPNMB Human, Sf9
Glycoprotein Nmb Human Recombinant, Sf9
GPNMB Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 462 amino acids (22-474a.a.) and having a molecular mass of 51.8kDa. GPNMB is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
Introduction
Glycoprotein Nmb (GPNMB), also known as osteoactivin, is a type I transmembrane glycoprotein. It was initially identified in a poorly metastatic melanoma cell line. GPNMB is partially localized to the cell surface, and its large N-domain can be released into the extracellular space through ectodomain shedding by ADAM10 .
Key Biological Properties: GPNMB is involved in various cellular processes, including cell differentiation, tumor progression, and tissue regeneration . It has anti-inflammatory and reparative functions and is neuroprotective in animal models of neurodegenerative diseases .
Expression Patterns: GPNMB is expressed in multiple tissues such as bone, kidney, and skeletal muscle . It is also found in melanocytes, osteoclasts, osteoblasts, and dendritic cells .
Tissue Distribution: GPNMB expression has been detected in various tissues, including the brain, where it is associated with neuroinflammatory responses in conditions like Alzheimer’s disease .
Primary Biological Functions: GPNMB plays a role in cell differentiation, tumor progression, and tissue regeneration . It also has anti-inflammatory and reparative functions .
Role in Immune Responses: GPNMB modulates innate and adaptive immunity by suppressing pro-inflammatory responses and promoting the recruitment and polarization of immunosuppressive cells .
Pathogen Recognition: GPNMB is involved in the neuroinflammatory response to pathogens, particularly in neurodegenerative diseases like Alzheimer’s disease .
Mechanisms with Other Molecules and Cells: GPNMB interacts with other molecules and cells through its transmembrane domain and ectodomain shedding . It can bind to the CD44 receptor on tumor cells, activating them to express cytokines like IL-33 and its receptor IL-1R1L .
Binding Partners: GPNMB binds to various receptors, including CD44, and interacts with molecules involved in inflammatory and reparative processes .
Downstream Signaling Cascades: GPNMB activates downstream signaling cascades that promote cell survival, differentiation, and tissue repair .
Transcriptional Regulation: The expression of GPNMB is regulated at the transcriptional level by various factors, including inflammatory cytokines .
Post-Translational Modifications: GPNMB undergoes post-translational modifications, such as ectodomain shedding by ADAM10, which releases its large N-domain into the extracellular space .
Biomedical Research: GPNMB is studied for its role in neurodegenerative diseases, cancer, and tissue regeneration .
Diagnostic Tools: GPNMB is being investigated as a potential biomarker for Alzheimer’s disease and other neurodegenerative conditions .
Therapeutic Strategies: GPNMB has therapeutic potential in treating neurodegenerative diseases, cancer, and inflammatory conditions due to its anti-inflammatory and reparative functions .
Development: GPNMB is involved in cell differentiation and tissue development .
Aging: GPNMB expression increases with age and is associated with age-related neuroinflammatory responses .
Disease: GPNMB plays a role in the progression of neurodegenerative diseases, cancer, and inflammatory conditions .
