Recombinant Proteins
Product List
SIRPA Human
Signal-Regulatory Protein Alpha Human Recombinant
SIRPA Human, HEK
Signal-Regulatory Protein Alpha Human Recombinant, HEK
SIRPA Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (27-373a.a) containing 356 amino acids and having a molecular mass of 39kDa.
SIRPA is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
SIRPA Rat
Signal-Regulatory Protein Alpha Rat Recombinant
SIRPB1 Human
Recombinant Human Signal Regulatory Protein Beta 1
SIRPB1produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (30-371 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 348 amino acids and having a molecular mass of 38.0kDa. SIRPB1shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
SIRPG Human, Sf9
Signal-Regulatory Protein Gamma Human Recombinant, Sf9
SIRPG Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 574 amino acids (29-360a.a.) and having a molecular mass of 64.0kDa. SIRPG is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
SIRPG Human
Signal-Regulatory Protein Gamma Human Recombinant
SIRPG is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Signal-Regulatory Proteins (SIRPs) are a family of transmembrane glycoproteins involved in immunological signaling, primarily expressed by myeloid cells . The SIRP family includes several members such as SIRPα, SIRPβ1, SIRPβ2, SIRPδ, and SIRPγ . These proteins are characterized by their extracellular immunoglobulin-like domains and their role in cell-cell communication .
Key Biological Properties: SIRPs are involved in various immunological processes, including the regulation of phagocytosis and immune cell signaling .
Expression Patterns: SIRPs are predominantly expressed in myeloid cells, including macrophages, dendritic cells, and neutrophils .
Tissue Distribution: SIRPs are found in various tissues, with high expression levels in the immune system, particularly in the spleen, lymph nodes, and bone marrow .
Primary Biological Functions: SIRPs play a crucial role in the regulation of immune responses, including the inhibition of phagocytosis and the modulation of cytokine production .
Role in Immune Responses: SIRPα, for example, interacts with CD47 to deliver a “don’t eat me” signal, preventing the phagocytosis of healthy cells by macrophages .
Pathogen Recognition: SIRPs are also involved in the recognition and clearance of pathogens by modulating the activity of immune cells .
Mechanisms with Other Molecules and Cells: SIRPs interact with various ligands, including CD47, to mediate their effects .
Binding Partners: The primary binding partner for SIRPα is CD47, which is ubiquitously expressed on cell surfaces .
Downstream Signaling Cascades: Upon binding to CD47, SIRPα recruits and activates protein tyrosine phosphatases such as SHP-1 and SHP-2, leading to the inhibition of phagocytosis and modulation of other immune responses .
Control of Expression and Activity: The expression and activity of SIRPs are regulated at multiple levels, including transcriptional and post-translational modifications .
Transcriptional Regulation: Various transcription factors and signaling pathways regulate the expression of SIRP genes .
Post-Translational Modifications: SIRPs undergo post-translational modifications such as phosphorylation, which can modulate their activity and interactions with other proteins .
Biomedical Research: SIRPs are studied for their role in immune regulation and potential therapeutic targets .
Diagnostic Tools: SIRP expression levels can serve as biomarkers for certain diseases, including cancers and autoimmune disorders .
Therapeutic Strategies: Targeting the CD47-SIRPα interaction is being explored as a therapeutic strategy for cancer, as blocking this interaction can enhance the phagocytosis of tumor cells by macrophages .
Development: SIRPs are involved in the development and differentiation of immune cells .
Aging: Changes in SIRP expression and function have been associated with aging and age-related diseases .
Disease: Dysregulation of SIRP signaling has been implicated in various diseases, including cancer, autoimmune disorders, and infectious diseases .
