SIRPG Human, Sf9
Sf9, Baculovirus cells.
Signal Regulatory Protein Gamma, CD172 Antigen-Like Family Member B, Signal-Regulatory Protein Beta-2, Signal-Regulatory Protein Gamma, CD172g Antigen, SIRP-Gamma, SIRP-B2, SIRPB2, Signal-Regulatory Protein Beta 2, SIRP-Beta-2, SIRP Beta 2, SIRPgamma, BA77C3.1, CD172g.
Greater than 90.0% as determined by SDS-PAGE.
Description
SIRPG Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 574 amino acids (29-360a.a.) and having a molecular mass of 64.0kDa. SIRPG is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Signal-Regulatory Protein Gamma, also known as SIRPG, is a member of the signal-regulatory protein (SIRP) family. SIRPs are receptor-type transmembrane glycoproteins known for their role in negatively regulating signaling processes associated with receptor tyrosine kinases. SIRPG is also classified as part of the immunoglobulin superfamily.
Recombinant human SIRPG, produced in Sf9 insect cells using a baculovirus expression system, is a single polypeptide chain with glycosylation. It comprises 574 amino acids, spanning from residue 29 to 360a.a., and exhibits a molecular weight of 64.0 kDa. The protein is engineered with a 239 amino acid hIgG-His tag fused at its C-terminus. Purification is achieved through proprietary chromatographic techniques.
The SIRPG protein solution is provided at a concentration of 0.5 mg/ml and is formulated in a Phosphate Buffered Saline (PBS) solution with a pH of 7.4 and supplemented with 20% glycerol.
The purity of the protein is determined to be greater than 90.0% based on SDS-PAGE analysis.
The biological activity of the protein, assessed by its binding affinity to human CD47 in a functional ELISA assay, exhibits an ED50 value of less than or equal to 1 ug/ml.
Signal Regulatory Protein Gamma, CD172 Antigen-Like Family Member B, Signal-Regulatory Protein Beta-2, Signal-Regulatory Protein Gamma, CD172g Antigen, SIRP-Gamma, SIRP-B2, SIRPB2, Signal-Regulatory Protein Beta 2, SIRP-Beta-2, SIRP Beta 2, SIRPgamma, BA77C3.1, CD172g.
Sf9, Baculovirus cells.
ADPEEELQMI QPEKLLLVTV GKTATLHCTV TSLLPVGPVL WFRGVGPGRE LIYNQKEGHF PRVTTVSDLT KRNNMDFSIR ISSITPADVG TYYCVKFRKG SPENVEFKSG PGTEMALGAK PSAPVVLGPA ARTTPEHTVS FTCESHGFSP RDITLKWFKN GNELSDFQTN VDPTGQSVAY SIRSTARVVL DPWDVRSQVI CEVAHVTLQG DPLRGTANLS EAIRVPPTLE VTQQPMRVGN QVNVTCQVRK FYPQSLQLTW SENGNVCQRE TASTLTENKD GTYNWTSWFL VNISDQRDDV VLTCQVKHDG QLAVSKRLAL EVTVHQKDQS SDATPLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
Signal-Regulatory Protein Gamma (SIRPG) is a member of the signal-regulatory protein (SIRP) family, which belongs to the immunoglobulin superfamily. These proteins are receptor-type transmembrane glycoproteins involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes .
The recombinant form of Signal-Regulatory Protein Gamma (Human Recombinant, Sf9) is produced using the Sf9 insect cell expression system. This system is widely used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells .
