SIRPB1 Human

Recombinant Human Signal Regulatory Protein Beta 1

Cat. No.

BT27355

Source

Sf9, Baculovirus cells.

Synonyms

SIRPB1, CD172b, SIRP-BETA-1, SIRP-Beta-1 Isoform 3, CD172b Antigen, CD172b.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

SIRPB1produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (30-371 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 348 amino acids and having a molecular mass of 38.0kDa. SIRPB1shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Product Specs

Introduction

Signal-regulatory protein beta-1 isoform 1, or SIRPB1, is an immunoglobulin-like cell surface receptor belonging to the SIRP superfamily. This receptor plays a role in the negative regulation of tyrosine kinase-coupled signaling pathways. SIRPB1 is involved in activating tyrosine kinase SYK and interacting with SIRP alpha molecules, which activate SHP-1 and SHP-2 protein tyrosine phosphatases.

Description

Produced in Sf9 Baculovirus cells, SIRPB1 is a single, glycosylated polypeptide chain (amino acids 30-371) with a 6-amino acid His Tag fused to the C-terminus. It comprises 348 amino acids, resulting in a molecular mass of 38.0 kDa. On SDS-PAGE under reducing conditions, SIRPB1 exhibits multiple bands between 40-57 kDa. It is purified using proprietary chromatographic techniques.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

SIRPB1 protein solution (0.25 mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.

Stability

For short-term storage (2-4 weeks), store at 4°C. For longer periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Greater than 90.0% purity as determined by SDS-PAGE analysis.

Synonyms

SIRPB1, CD172b, SIRP-BETA-1, SIRP-Beta-1 Isoform 3, CD172b Antigen, CD172b.

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

EDELQVIQPE KSVSVAAGES ATLRCAMTSL IPVGPIMWFR GAGAGRELIY NQKEGHFPRV TTVSELTKRN NLDFSISISN ITPADAGTYY CVKFRKGSPD DVEFKSGAGT ELSVRAKPSA PVVSGPAVRA TPEHTVSFTC ESHGFSPRDI TLKWFKNGNE LSDFQTNVDP AGDSVSYSIH STARVVLTRG DVHSQVICEI AHITLQGDPL RGTANLSEAI RVPPTLEVTQ QPMRAENQAN VTCQVSNFYP RGLQLTWLEN GNVSRTETAS TLIENKDGTY NWMSWLLVNT CAHRDDVVLT CQVEHDGQQA VSKSYALEIS AHQKEHGSDI THEAALAPTA PLHHHHHH.

Product Science Overview

Structure and Function

SIRPB1 is an immunoglobulin-like cell surface receptor that plays a crucial role in the negative regulation of receptor tyrosine kinase-coupled signaling processes. It participates in the recruitment of tyrosine kinase SYK and triggers the activation of myeloid cells when associated with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs .

Genetic Information

The SIRPB1 gene is located on chromosome 20 and encodes multiple transcript variants that produce different isoforms of the protein. The gene is also known by several aliases, including CD172b and SIRP-Beta-1.

Recombinant Human SIRPB1

Recombinant Human SIRPB1 is produced using human embryonic kidney cells (HEK293) and is available in a carrier-free form. The recombinant protein is typically used in research to study its binding ability and functional properties. It is purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE.

Applications in Research

Recombinant Human SIRPB1 is used in various research applications, including studies on cell signaling, immune response, and receptor-ligand interactions. Its ability to bind with other proteins, such as SP-D, is measured using functional ELISA assays.

Clinical Relevance

SIRPB1 has been associated with several diseases, including hereditary essential tremor and intracranial berry aneurysm. Its role in the immune system and cell signaling makes it a potential target for therapeutic interventions in immune-related disorders and cancers.

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SIRPB1 Human

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