Recombinant Proteins
Product List
NPM1 Human
Nucleophosmin Human Recombinant
NPM1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NPM2 Human
Nucleophosmin 2 Human Recombinant
NPM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23, numatrin, or NPM1, is an abundant nucleolar protein found in the nuclei of proliferating cells . It is involved in various cellular processes, including ribosome biogenesis, mRNA processing, chromatin remodeling, and embryogenesis . NPM1 is classified as a multifunctional protein due to its diverse roles in cellular biology .
Key Biological Properties: NPM1 is a critical cellular protein that participates in mRNA transport, chromatin remodeling, apoptosis, and genome stability . It functions as both a proto-oncogene and a tumor suppressor .
Expression Patterns: NPM1 is abundantly expressed in all tissues, with higher expression levels in proliferating cells .
Tissue Distribution: NPM1 is predominantly localized in the nucleolus but can also be found in the nucleoplasm and cytoplasm under certain conditions .
Primary Biological Functions: NPM1 is involved in ribosome biogenesis, mRNA processing, chromatin remodeling, and embryogenesis . It also plays a crucial role in maintaining genomic stability by participating in various DNA repair pathways and regulating apoptosis .
Role in Immune Responses and Pathogen Recognition: NPM1 is implicated in immune responses and pathogen recognition, although its exact role in these processes is still being studied .
Mechanisms with Other Molecules and Cells: NPM1 interacts with various molecules and cells, including histones and other chromatin remodeling proteins . It binds to single-stranded and double-stranded nucleic acids, with a preference for G-quadruplex forming nucleic acids .
Binding Partners: NPM1 has multiple binding partners, including ribonucleoprotein structures and viral basic proteins .
Downstream Signaling Cascades: NPM1 is involved in several downstream signaling cascades that regulate apoptosis, DNA repair, and chromatin remodeling .
Expression and Activity Control: The expression and activity of NPM1 are regulated through various mechanisms, including transcriptional regulation and post-translational modifications .
Transcriptional Regulation: NPM1 expression is controlled at the transcriptional level by various transcription factors .
Post-Translational Modifications: NPM1 undergoes several post-translational modifications, including phosphorylation, acetylation, and SUMOylation, which regulate its activity and localization .
Biomedical Research: NPM1 is widely studied in biomedical research due to its multifunctional roles and involvement in various cellular processes .
Diagnostic Tools: NPM1 is used as a diagnostic marker for certain cancers, particularly acute myeloid leukemia (AML), where NPM1 mutations are common .
Therapeutic Strategies: Targeting NPM1 and its pathways is being explored as a therapeutic strategy for treating cancers and other diseases .
