NPM1 Human
Nucleophosmin Human Recombinant
Cat. No.
BT20466
Source
Escherichia Coli.
Synonyms
Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM1, NPM, B23.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
NPM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-294 a.a) and having a molecular mass of 34.7kDa.
NPM1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NPM1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Nucleophosmin (NPM1) is a phosphoprotein that moves between the nucleus and the cytoplasm of a cell. It is believed to be involved in several cellular processes, including the regulation of the ARF/p53 pathway. NPM1 is known to interact with other genes, forming fusion partners, notably with the anaplastic lymphoma kinase gene found on chromosome 2. Mutations in the NPM1 gene are associated with acute myeloid leukemia.
Description
Recombinant human NPM1, produced in E. coli bacteria, is a single polypeptide chain that lacks glycosylation. It consists of 314 amino acids (specifically, amino acids 1 to 294), resulting in a molecular weight of 34.7 kDa. The NPM1 protein has a 20 amino acid His-tag attached to its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear and sterile solution.
Formulation
The NPM1 protein is provided in a solution with a concentration of 0.5 mg/ml. The solution contains 20 mM Tris-HCl buffer at a pH of 8.0, 10% glycerol, 0.15 M NaCl (sodium chloride), and 1 mM DTT (dithiothreitol).
Stability
For short-term storage (up to 2-4 weeks), the NPM1 protein solution should be kept at 4°C. For longer storage, it is recommended to freeze the solution at -20°C. To further enhance long-term stability, consider adding a carrier protein such as HSA or BSA (0.1% concentration). It is important to avoid repeated freezing and thawing of the protein solution.
Purity
The purity of the NPM1 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM1, NPM, B23.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIV EAEAMNYEGS PIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEE EDVKLLSISG KRSAPGGGSK VPQKKVKLAA
DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL.
DEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTP AKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAK MQASIEKGGS LPKVEAKFIN YVKNCFRMTD QEAIQDLWQW RKSL.
Product Science Overview
Gene Organization and Evolutionary History
The human NPM1 gene is located on chromosome 5q35 and is composed of 12 exons . It encodes at least two isoforms: NPM1.1 (or B23.1), which is the full-length transcript resulting in a 294 amino acids protein, and NPM1.3 (or B23.2), which lacks the last 35 amino acids of the NPM1 C-terminus . These isoforms have different expression levels and localization within the cell .
Functions of Nucleophosmin
Nucleophosmin is involved in several critical cellular functions:
- Ribosome Biogenesis: NPM1 is essential for the assembly and transport of ribosomes .
- mRNA Processing: It plays a role in the processing and transport of mRNA within the cell .
- Chromatin Remodeling: NPM1 interacts with chromatin, binding to histones and other chromatin remodeling proteins .
- Embryogenesis: It is involved in the early stages of embryonic development .
- DNA Repair and Apoptosis: NPM1 is crucial for maintaining genomic stability by participating in various DNA repair pathways and regulating apoptosis .
Role in Disease
NPM1 is frequently overexpressed, mutated, rearranged, and deleted in human cancers . It can function both as a proto-oncogene and a tumor suppressor . Mutations in the NPM1 gene, particularly those leading to the expression of a cytoplasmic mutant protein (NPMc+), are common in acute myeloid leukemia (AML) . These mutations are associated with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile, and a favorable prognosis .
Recombinant Human Nucleophosmin
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