NPM2 Human
Nucleophosmin 2 Human Recombinant
Cat. No.
BT20572
Source
E.coli.
Synonyms
Nucleophosmin/nucleoplasmin 2, nucleoplasmin-2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
NPM2 Human Recombinant produced in E. coli is a single polypeptide chain containing 237 amino acids (1-214) and having a molecular mass of 26.6 kDa.
NPM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
NPM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
NPM2, a member of the nucleoplasmin family, serves as a core histones chaperone involved in chromatin reprogramming. Its role is particularly crucial during fertilization and early embryonic development. NPM2 also plays a part in sperm DNA decondensation during fertilization.
Description
Recombinant NPM2 Human, produced in E. coli, is a single polypeptide chain consisting of 237 amino acids (1-214) with a molecular mass of 26.6 kDa. It comprises NPM2 fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, filtered, and colorless solution.
Formulation
The NPM2 solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT, and 20% glycerol.
Stability
For optimal storage, keep the solution at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Purity
The purity is determined to be greater than 85% by SDS-PAGE.
Synonyms
Nucleophosmin/nucleoplasmin 2, nucleoplasmin-2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNLSSAS STEEKAVTTV LWGCELSQER RTWTFRPQLE GKQSCRLLLH TICLGEKAKE EMHRVEILPP ANQEDKKMQP VTIASLQASV LPMVSMVGVQ LSPPVTFQLR AGSGPVFLSG QERYEASDLT WEEEEEEEGE EEEEEEEDDE DEDADISLEE
QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK
QSPVKQVKRL VPQKQASVAK KKKLEKEEEE IRASVRDKSP VKKAKATARA KKPGFKK
Product Science Overview
Gene Organization and Isoforms
The human NPM1 gene is located on chromosome 5q35 and is composed of 12 exons . It encodes at least two isoforms:
- NPM1.1 (B23.1): The full-length transcript, resulting in a 294 amino acids protein (35–40 kDa), abundantly expressed in all tissues .
- NPM1.3 (B23.2): Results from the use of a distinct 3′ exon, encoding a protein expressed at low levels in cells, lacking the last 35 amino acids of the NPM1 C-terminus .
Functions of Nucleophosmin
NPM1 is a multifunctional protein involved in several biological processes:
- Ribosome Biogenesis: NPM1 plays a crucial role in the assembly and transport of ribosomes .
- mRNA Processing: It is involved in the processing and transport of mRNA .
- Chromatin Remodeling: NPM1 interacts with chromatin, binding to histones and other chromatin remodeling proteins .
- Apoptosis Regulation: It regulates apoptosis, contributing to cellular homeostasis .
- DNA Repair: NPM1 is involved in various DNA repair pathways, maintaining genomic stability .
NPM1 in Disease
NPM1 is frequently overexpressed, mutated, rearranged, and deleted in human cancers . Mutations in the NPM1 gene, leading to the expression of a cytoplasmic mutant protein (NPMc+), are the most common genetic abnormalities found in acute myeloid leukemias (AML) . AML with mutated NPM1 has distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile, and clinically, a better response to induction therapy and a favorable prognosis .
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