Recombinant Proteins
Product List
NUP210 Human
Nucleopurin 210kDa Recombinant Human
Recombinant NUP210 protein is a construct carrying multiple concatenated copies of the short cytoplasmic gp210 C-terminus (which contains the autoreactive epitopes) and having a molecular mass of 27kDa (pH 9.8). NUP210 protein is fused to a hexa-histidine purification tag.
NUP62 Human
Nucleopurin 62kDa Human Recombinant
NUP62 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66 kDa.
NUP62 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
NUP62CL Human
Nucleopurin 62kDa C-Terminal Like Human Recombinant
NUP62CL is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Nucleoporins are a family of proteins that constitute the nuclear pore complex (NPC), which is embedded in the nuclear envelope of eukaryotic cells. The NPC regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are classified based on their structural and functional roles within the NPC. They include scaffold nucleoporins, which form the structural framework, and FG-nucleoporins, which contain phenylalanine-glycine repeats and are involved in transport processes .
Key Biological Properties: Nucleoporins are essential for nucleocytoplasmic transport, facilitating the movement of proteins, RNA, and other molecules across the nuclear envelope. They exhibit high transport efficiency, with a single NPC capable of transporting up to 60,000 protein molecules per minute .
Expression Patterns and Tissue Distribution: Nucleoporins are ubiquitously expressed in eukaryotic cells, with variations in expression levels depending on the cell type and developmental stage. Certain nucleoporins, such as Nup62, are more abundant and play critical roles in various tissues .
Primary Biological Functions: Nucleoporins are integral to the NPC’s function, mediating the bidirectional transport of macromolecules between the nucleus and cytoplasm. They also play roles in chromatin organization, gene expression regulation, and cell cycle progression .
Role in Immune Responses and Pathogen Recognition: Nucleoporins are involved in immune responses by regulating the transport of immune signaling molecules and transcription factors. They also participate in the recognition and response to pathogens by facilitating the nuclear import of pathogen-associated molecular patterns .
Mechanisms with Other Molecules and Cells: Nucleoporins interact with transport receptors such as importins and exportins, which recognize nuclear localization signals (NLS) on cargo molecules. This interaction facilitates the transport of cargo through the NPC .
Binding Partners and Downstream Signaling Cascades: Nucleoporins bind to various proteins and RNA molecules, influencing downstream signaling pathways. For example, they interact with the Wnt/β-catenin signaling pathway, which is crucial for cell differentiation and development .
Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of nucleoporins are regulated at multiple levels, including transcriptional regulation and post-translational modifications. Specific transcription factors and signaling pathways modulate nucleoporin gene expression .
Transcriptional Regulation and Post-Translational Modifications: Nucleoporins undergo various post-translational modifications, such as phosphorylation and ubiquitination, which affect their stability, localization, and function. These modifications are crucial for the dynamic regulation of NPC activity .
Biomedical Research: Nucleoporins are studied extensively in biomedical research to understand their roles in cellular processes and disease mechanisms. They serve as models for studying nucleocytoplasmic transport and its regulation .
Diagnostic Tools and Therapeutic Strategies: Nucleoporins have potential applications in diagnostics and therapeutics. Alterations in nucleoporin expression or function are associated with various diseases, including cancer and neurodegenerative disorders. Targeting nucleoporins or their regulatory pathways could provide novel therapeutic strategies .
Role Throughout the Life Cycle: Nucleoporins play critical roles throughout the life cycle, from development to aging and disease. During development, they are involved in cell differentiation and organogenesis. In aging, changes in nucleoporin function can affect cellular homeostasis and contribute to age-related diseases .
From Development to Aging and Disease: Nucleoporins are essential for maintaining cellular function and integrity. Mutations or dysregulation of nucleoporins can lead to developmental defects, impaired immune responses, and increased susceptibility to diseases .
