NUP62 Human
Greater than 95% as determined by SDS-PAGE.
Description
NUP62 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66 kDa.
NUP62 is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Provides a concise overview of Nucleopurin 62kDa (NUP62), its function, and its significance in cellular processes and disease.
Describes the specific characteristics of our recombinant NUP62 protein, including its production host, molecular weight, and purification tag.
Specifies the visual appearance of the product as supplied to the customer.
Details the composition of the buffer solution in which the NUP62 protein is provided.
Provides guidelines for storing the product to maintain its quality and activity over time.
Indicates the percentage of the target protein (NUP62) in the product, determined by SDS-PAGE analysis.
Recommends a range of concentrations for coating ELISA plates with NUP62, noting that the optimal concentration may vary.
Suggests a specific application for NUP62, highlighting its compatibility with Western blot analysis using anti-Nup62 antibodies.
Describes the immunological properties of NUP62, particularly its ability to interact with human antibodies and its utility in ELISA and immunodot assays.
Product Science Overview
NUP62 is a glycosylated polypeptide chain with a calculated molecular mass of approximately 54,626 Daltons . It is expressed with a 10x His tag at the N-terminus, which facilitates its purification through chromatographic techniques . The protein is produced in SF9 insect cells, which are commonly used for the expression of recombinant proteins due to their ability to perform post-translational modifications similar to those in mammalian cells .
NUP62 plays a pivotal role in the NPC by forming a part of the central channel through which nucleocytoplasmic transport occurs. It interacts with other nucleoporins to create a selective barrier that allows the passage of specific molecules while preventing the free diffusion of others. This selectivity is essential for maintaining cellular homeostasis and regulating gene expression.
The recombinant NUP62 protein is typically produced using baculovirus expression systems in SF9 insect cells. The process involves the following steps:
- Cloning: The gene encoding NUP62 is cloned into a baculovirus transfer vector, which is then used to transfect SF9 cells.
- Expression: The transfected cells are infected with recombinant baculovirus, leading to the expression of NUP62.
- Purification: The expressed protein is purified using affinity chromatography, taking advantage of the His tag at the N-terminus. This tag binds to nickel ions, allowing the protein to be isolated from other cellular components .
Recombinant NUP62 is used in various research applications to study the structure and function of the NPC. It is also employed in the investigation of diseases associated with defects in nucleocytoplasmic transport, such as certain cancers and neurodegenerative disorders. Understanding the role of NUP62 in these processes can provide insights into potential therapeutic targets and strategies.
